Drug screening simplified
Powerful UHPLC-MSn analysis
Toxtyper® delivers a robust and automated drug testing solution based on the fastest LC-MSn technology in the market, the amaZon speed. The Toxtyper® provides a combination of high-performance LC-MS/MS and confident identification with full transferability of library search results from lab to lab.
The Toxtyper® solution was developed to meet the needs of forensic and clinical research labs. The unique patented SmartFrag technology virtually removes any variation and hence the need for tuning from the MS/MS process.
The intuitive, easy-to-use Toxtyper® user interface is designed specifically for non-MS experts. Its push-button sample analysis is ideal for a multi-user environment and 24/7 operation.
Key attributes of the Toxtyper®:
The Toxtyper® holds a curated high quality spectral library with approximately 3000 MSn spectra of nearly 1200 compounds of research toxicology interest. It also offers a smaller targeted library for drugs of abuse (DOA) screening research. The Toxtyper® supports the Maurer/Wissenbach/Weber LC-MSn library* containing almost 6000 drugs and metabolites.
*Maurer/Wissenbach/Weber LC-MSn Library of Drugs, Poisons and Their Metabolites 2nd Edition; ISBN: 978-3-527-34649
Open library concept
Recent increases in drug abuse by new psychoactive substances (NPS), such as synthetic cannabinoids, constantly change their analytical requirements, meaning screening methods must be rapidly adapted.
The Toxtyper® libraries are constantly updated with contributions from customers and collaboration partners. User-defined library entries can be created quickly and easily, allowing laboratories to respond rapidly to new patterns of drug abuse.
Easy-to-use software designed for non-MS experts
The Toxtyper® software enables one-click review of library search results for fast interactive results evaluation using chromatogram and MSn spectra views.
Identified compounds can be sorted by various parameters, including signal intensity, retention time, or alphabetically. Color coding facilitates the review process.
Toxtyper®: a complete solution for clinical research and forensic toxicology
Today's forensic toxicology and clinical research labs are challenged by demands for fast and accurate results, along with lab efficiency and cost reduction. Further pressure results from the continuous emergence of new drugs into the market, requiring frequent updates of sample screening methods.
The Toxtyper® LC-MSn approach with library searching is an easy-to-use, flexible, and reliable screening solution for modern toxicology research.
Reproducibility and transferability through SmartFrag
Data-dependent MSn acquisition using Bruker’s patented SmartFrag technology enables the successful analysis of different types of compounds, regardless of their chemical nature, concentration, or sample matrix. The resulting MSn spectra and identification results are completely reproducible and transferable from instrument to instrument and lab to lab.
From comprehensive screening to semi-quantification:
Different well-established sample preparation methods, such as liquid-liquid extraction (LLE), solid-phase extraction (SPE) or simple protein precipitation, can be used within the Toxtyper® workflow. Protein precipitation is quick and easy and is well suited to the comprehensive analysis of a wide range of analytes.
One example is to demonstrate the simultaneous, highly sensitive detection of drugs in human urine. Following simple sample preparation (liquid-liquid-extraction) of 1 mL of urine, the Toxtyper® permits detection down to 0.8 ng/mL.
Tailor-made sub-libraries for increased sensitivity
The Toxtyper® provides the option to generate smaller libraries for specific needs.
Screening, confirmation, and semi-quantitation in one run
To add value to the comprehensive screening, the standard Toxtyper® analysis using Toxtyper® and DOA libraries can be extended by semi-quantification.
After performing a one-point calibration for the target compounds using two or more internal standards, the subsequent Toxtyper® analyses provide qualitative screening results and semiquantitive values, e.g. concentration above or below a target value.
Although drug analysis using immunological methods is fast, it is not always reliable. The available screening tests can easily lead to false positive results. For the confirmation of such immunoassay results, complex and time-consuming GC-MS analysis has been so far performed by the Stuttgart hospital.
Since March 2017, the Toxtyper® system has been used for the screening of drugs and toxins in blood, oral fluid and urine. Without time-consuming sample preparation, Toxtyper® delivers fast and reliable results that are highly specific and reproducible. In addition, the Toxtyper® provides a high degree of safety in toxicological analysis prior to the neurological diagnosis of irreversible brain failure.
A 34-year-old patient with dizziness, nausea and vomiting was admitted to the emergency room. The anamnesis suggests consumption of drugs, but the patient denies the use of illegal drugs. Urine screening using various immunoassays was negative for Amphetamines, Barbiturates, Benzodiapezepins, Cocaine, Opiates and Cannabis. The Toxtyper® identified Mitragynin and several metabolites of Paynanthein and Speciociliantin, which are alkaloids present in leaves and fruits of the Kratom tree. Kratom is used for substitution of Opioids and is not regulated by German narcotics law.
“The Toxtyper® is our fast routine workhorse solution for General Unknown Screening (GUS) in diverse matrices such as blood, urine or gastric content. We answer demanding questions for our addictions lab customers by new Toxtyper® applications like GUS in saliva.“
Benefits of using Toxtyper®: "Streamlined sample throughput in our laboratory by unified, faster and easier sample preparation for urine samples and 24/7 usage of the system. Ready to use methods combined with comprehensive libraries."
For Research Use Only. Not for use in clinical diagnostic procedures.