amaZon speed ETD Ion Trap

Unübertroffene Ionenfallen-Leistung für Proteomik-Lösungen

Die neue amaZon speed ETD integriert Schlüsselerfindungen für beispiellose Proteomik-Funktionen. Der hohe dynamische Bereich und die Komplexität von Proteomik-Proben aufgrund von Modifikationen nach der Übersetzung erfordern maximale Geschwindigkeit und Empfindlichkeit, um so viele Peptide wie möglich zu erfassen.

Die Ionenfalle amaZon speed ETD setzt dank ihrer verbesserten MS/MS-Geschwindigkeit, der neuen SWIFT-Isolierung und der SMART-Fragmentierung neue Standards in der Protein-Identifikation. Dies resultiert in einer Routine-Identifikation von -2500 eindeutig identifizierten Proteinen in einem einzigen LC/MS/MS-Durchlauf von 5 µg HeLa-Lysat. Die duale Ionentrichter-Transferleitung liefert absolute Benchmark-Empfindlichkeit.

Unmatched ion trap performance through innovation

  • Bottom-up proteomics identifying more low abundant proteins due to greatly increased MS/MS duty cycle
  • Top-down sequencing (TDS) for highest protein sequence coverage
  • Highest confidence through market leading mass accuracy
  • Leading dual ion funnel technology for unparalleled sensitivity
  • Outstanding ETD/PTR applications for full PTM analysis
  • New SMART isolation and fragmentation
  • Unrivalled scan speed of 52,000 u/sec at a peak width < 0.5 u for resolution of doubly charged ions

Applications amaZon speed ETD

High Sequence Coverage by CaptiveSpray™

The amaZon speed is equipped with the high-transmission, robust CaptiveSpray™ source for highest performance and reproducibility in bottom-up studies.

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Ultimate protein identification rates

Suvey view of a Hela cell digest (5µg) separated by a nano UHPLC and amaZon speed ETD equipped with a CaptiveSpray™ ion source.

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Market leading dynamic range

The amaZon speed ETD identifies proteins over at least 5 concentration levels  in a single run.

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Complete PTM characterization by Electron Transfer Dissociation (ETD)

Many proteins execute their molecular function by reversible posttranslational modifications (PTMs) like e.g. phosphorylation. The identification and unambiguous localization of the modification site is of major importance to understand the biological context. ETD preserves the modification of the peptide backbone and therefore allows the clear assignment of the modification site. The amaZon speed is the unrivalled performance leader by its market proven ETD technology: most sensitive, robust and reliable. 

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Bruker Daltonics services are performed by factory trained and certified engineers. DIN EN ISO 9001:2000 requirements are fulfilled.

 

Available services include:

  • Help desk service for a faster access to our support team, to your service order follow up and for general information
  • Planned maintenance, according to our requirement
  • Telephone support from highly-skilled troubleshooting professionals, to isolate and resolve hard- and software problems
  • Web-based remote instrument service for service diagnosis and applications support
  • Customer on-site repair and maintenance service
  • Spare parts availability typically over night or within a few working days worldwide
  • Compliance services for installation qualification, operational qualification / performance verification
  • Site planning and relocation
  • Advise to improve labs efficiency (consumable, method development & how to run the complete solution)

For Research Use Only. Not for Use in Clinical Diagnostic Procedures.