Bruker at Cell Bio 2025
Visit Us at Booth 1001
December 6-10 | Pennsylvania Convention Center | Philadelphia, PA
Connect with Bruker experts and explore our advanced microscopy solutions for life science imaging and analysis, including:
- Super-Resolution Microscopy for nanoscale quantitative imaging
- Light-Sheet Microscopes for long-term developmental and dynamic cell studies
- BioAFM for high-resolution imaging and nanoscale biomechanics
- Confocal Platforms for advanced live-cell imaging
Our solutions help scientists accelerate discoveries across cellular biology and beyond. Our team will be available throughout the show to discuss applications, answer questions, and help you find the right solutions for your research. See you there!
Conference Host:
American Society for Cell Biology (ASCB)
European Molecular Biology Organization (EMBO)
Conference Venue:
Pennsylvania Convention Center | Philadelphia, PA, USA
Don’t Miss Our Presentations
This year, we are giving several tech talks—stop by to hear case studies, technical insights, and application highlights from imaging experts.
Exhibitor Tech Talks
Imaging of Spheroids and Organoids Using High-content Screening and Light-sheet Microscopy
Speakers: Chris English
Date: Saturday, December 6, 2025
Time: 11:15 AM – 12:00 PM
Abstract: Life happens in a 3-dimensional world. To study this, Bruker offers a suite of fluorescence microscopy tools to enable research of biological processes on scales ranging from individual cells, organoids, tumors, and even entire organisms. In this presentation, we will focus our discussion on two different approaches, imaging of spheroids and organoids using Luxendo Light-Sheet microscopes and the Acquifer IM Automated Microscopy platform for high-content screening. After discussing the technology, aspects of the optomechanical design, and imaging applications, we will illustrate a workflow for identifying and characterizing drug effects on spheroids/organoids using the Acquifer IM screening microscope. Promising drugs can then be further evaluated on the Luxendo TruLive3D light-sheet microscope, enabling the acquisition of time-lapse datasets with high spatiotemporal resolution for detailed visualization and analysis of drug-induced phenotypes. Concerned with how to handle the large amounts of data created with these microscopes? Stick around for a discussion of the capabilities of our Acquifer HIVE Data Solution for the safe storage, reliable and fast access and powerful processing of your imaging, sequencing, and omics data.
Advanced AFM Imaging and Analysis of Cellular and Tissue Samples: Innovations in Large-Area Mapping and Mechanobiology
Speakers: Yi Wei
Date: Sunday, December 7, 2025
Time: 10:15 AM – 11:00 AM
Abstract: Atomic Force Microscopy (AFM) is crucial for nanoscale mechanical property mapping, offering high-resolution characterization of stiffness, adhesion, and viscoelasticity. This capability is essential for understanding material behavior in complex structures like living cells, tissues, and biomaterials, thereby driving forward studies of cell behavior, disease progression, and drug treatments. However, challenges such as sample roughness and limited lateral scanning range often hinder large-scale mechanical mapping, particularly for complex and heterogeneous specimens.
We have developed a new concept for AFM imaging, incorporating SmartMapping technology and CellWizard technology. SmartMapping coordinates AFM head motors and XYZ-piezo movement of the AFM stage. This innovation enables continuous, high-resolution automated mapping over extensive areas without user intervention, enhancing the precision and efficiency of AFM. Building on top of SmartMapping, the CellWizard hardware expands the application scinarios and incorporates multi-chamber sample compatibilty, accommodating double slides, multi-well plates, and petri dishes. In addition, the CellWizard software integrates with CellPose, a deep learning algorithm for cellular segmentation in microscopy images, enabling fully automated cell detection, measurements and analysis with pre-trained and user-defined models on AFM platforms. Our research leverages this newly developed AFM technology to perform multi-compartment imaging with high reproducibility and throughput across multiple regions.
In our studies, Cytochalasin D treated 3T3 fibroblasts were compared to non-treated controls, revealing significant time-lapse differences in mechanics, structure, and cytoskeletal organization. We further investigated highly corrugated 3D spheroid SKOV-3 model lines and zebrafish tumors exceeding 100 µm and 300 µm in height respectively, focusing on their structural and mechanical characteristics. By mapping mouse brain tissue along the anterior-posterior axis we draw functional correlations between the regional structural and mechanical differences and the underlying anatomical composition and architecture. Neuroblastoma tumors, 600 µm thick and embedded in low-melting agarose gels, were effectively analyzed despite their roughness, demonstrating the stage's capability to handle complex tissue samples.
The integration of these measurements within the context of our innovative AFM stage underscores the potential for large-area imaging and mapping in mechanobiology. Our work aims to advance the understanding of cellular and tissue mechanics, contributing to the broader field of mechanobiology and paving the way for future research and applications.
Characterizing membrane-associated molecular interactions directly on cells using single-cell Interaction Cytometry
Speakers: Matthew Peterfreund
Date: Sunday, December 7, 2025
Time: 10:15 AM – 11:00 AM
Abstract: Key processes such as membrane protein internalization or signal transduction depend on precise molecular interactions between molecules and membrane receptors. Binding kinetics of these molecular interactions are often investigated using isolated proteins, neglecting the membrane context of the target. However, the cellular membrane constitutes a complex microenvironment for embedded receptors, with factors like target mobility, co-receptors, and transmembrane domain folding affecting their behavior. Thus, understanding the influence of the cell membrane context on these interactions is pivotal for understanding molecular processes at the cell membrane. In this study, we present single-cell Interaction Cytometry (scIC), a novel technology to investigate how the native receptor environment influences the kinetic rates of molecules interacting with membrane targets. In scIC measurements, single cells are captured in polymer cages on the surface of a microfluidic chip, enabling the real-time measurement of the association and dissociation of fluorescently labelled analytes. We used antibodies as case studies and first investigated the binding behavior of anti-CD3-antibodies to Jurkat cells. Interestingly, differential binding behaviors could be observed on living versus fixed cells. Living cells showed an increase in avidity-based binding, which could be ascribed to the maintained mobility of the target receptors within the membrane. Next, we examined the binding of two therapeutic antibodies, pertuzumab and trastuzumab, which target distinct HER2 epitopes in HER2-overexpressing breast cancer cells and exhibit different clinical efficacies. We detected differences in their binding behavior with pertuzumab exhibiting faster association to the HER2 receptor compared to trastuzumab. Furthermore, we compared breast cancer cell lines with high and low HER2 expression levels, showing that target expression levels influence the residence time.
The observed differences in kinetic rates across varying membrane environments emphasize the need to consider the native cellular context when studying membrane-associated interactions. This insight is crucial not only for advancing our understanding of membrane biology but also for guiding the rational design of therapeutics targeting membrane proteins.
Meet Us at the Show
Throughout the event, our team will be at the booth showcasing our latest technology and will be available to answer your questions.
Schedule a meeting with us to discuss your unique measurement needs and challenges and find out how Bruker technology can advance your materials research.
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