Star Light, Star Bright: Considerations for Imaging Astrocyte Dynamics
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Unraveling the role of astrocytes in sleep regulation
During this on-demand webinar, Ashley Ingiosi, Ph.D., discusses her work using one-photon (1P) and two-photon (2P) microscopy to explore sleep, a prevalent health issue. Learn how Inscopix miniscope and Bruker’s multiphoton technology were used for in vivo calcium imaging of astrocytes in freely-behaving mice.
Presenter’s Abstract
Astrocytes play a big role in sleep-wake function and sleep homeostasis. During this talk, I will unpack how I overcame some of the major challenges associated with astroglial 1P and 2P imaging and analyses. Glia do not behave like neurons, and I will offer critical insights for anyone who wishes to record miniscope imaging data in astrocytes in freely behaving animals.
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Speakers
Ashley Ingiosi, Ph.D.
Postdoctoral Fellow, Frank Laboratory, Department of Biomedical Sciences, Washington State University