The study introduces a high-throughput microflow liquid chromatography mass spectrometry (LC–MS) setup, specifically using the timsTOF HT platform equipped with VIP HESI, a vacuum insulated probe heated electrospray ionization source. Johanna Tüshaus et al., established the workflow and created a protein expression map of 13 distinct brain regions, encompassing over 11,000 proteins. This resource uncovers brain region-enriched protein expression patterns, functional protein classes, and differences in protein localization among brain regions. A dedicated R Shiny app facilitates data exploration. Key findings include the scalability, robustness, and sensitivity of the microflow LC–MS approach and identification of unique proteomic fingerprints for each brain region.
Key Learnings:
"Planning a large-scale proteomic profiling project, we were in need of a fast, sensitive and robust LC-MS/MS setup. Coupling our well-established micro-flow LC technology via the VIP-HESI source to the timsTOF HT turned out to be a powerful combination. It enables deep proteome coverage using short gradients thereby linking speed with sensitivity. The development of the 50 µm ESI emitter was key to boost the sensitivity of our micro-flow LC timsTOF setup."
For Research Use Only. Not for use in clinical diagnostic procedures.