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Light-Sheet Microscopes

Webinar Recap:
Imaging Complex Structure with Luxendo Light-Sheet Microscopy and CLARITY Tissue Clearing

Read about light microscopy techniques for imaging large or dense biological samples

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KEYWORDS:
Organoids; Case Study; Cleared Sample Imaging; Tissue Clearing; CLARITY; Refractive Index Equilibrium; LSFM; SPIM

Using conventional light microscopy techniques to image large or dense biological samples can be challenging due to light scattering properties of tissues and mismatched refractive indices. The combination of tissue clearing and light-sheet microscopy enables fast, gentle, and high-resolution imaging deep within thick samples.

In this recap of the webinar presented by Sharla White, Ph.D., Vice President of Research and Development at ClearLight Bio, the practical applications and best-known methods of combined light-sheet fluorescence microscopy (LSFM) and CLARITY tissue clearing are presented. An example of CLARITY tissue clearing on mouse intestines is also presented, showcasing how imaging with LSFM provides added insight to the unique structure, shape, and layers of the intestine.

 

Readers can expect to learn more about:

  • Why tissue clearing is necessary for imaging thick and complex samples, such as tissue sections or organoids thicker than 100 µm;
  • How to implement the aqueous and hydrogel-based clearing technique, CLARITY, for the maintenance of structure as light-scattering components are removed; and
  • How and why the MuVi SPIM light-sheet microscope is used for imaging cleared tissues and organoids.

 

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