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KEYWORDS: Organoids; Case Study; Cleared Sample Imaging; Tissue Clearing; CLARITY; Refractive Index Equilibrium; LSFM; SPIM
Using conventional light microscopy techniques to image large or dense biological samples can be challenging due to light scattering properties of tissues and mismatched refractive indices. The combination of tissue clearing and light-sheet microscopy enables fast, gentle, and high-resolution imaging deep within thick samples.
In this recap of the webinar presented by Sharla White, Ph.D., Vice President of Research and Development at ClearLight Bio, the practical applications and best-known methods of combined light-sheet fluorescence microscopy (LSFM) and CLARITY tissue clearing are presented. An example of CLARITY tissue clearing on mouse intestines is also presented, showcasing how imaging with LSFM provides added insight to the unique structure, shape, and layers of the intestine.