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Multiphoton Microscope Modules

Rotating Nosepiece

The most flexible solution for high-precision off-axis multiphoton imaging

About Bruker's Rotating Nosepiece Module

Bruker's integrated rotating nosepiece module facilitates off-axis multiphoton microscopy imaging. Consequently, this nosepiece enables multiphoton microscope users to image areas of a specimen that are not accessible from the vertical axis and supports high-efficiency signal collection across sample types.

This type of high-precision off-axis imaging proves to be essential for a wide variety of imaging studies as it provides both of the following:

  1. The capture of structures of interest without moving out of the field of view, even when aligning with continuous structures that lie along a particular axis.
  2. The reduction of light scatter that could otherwise reduce the depth of penetration and lead to low contrast imaging — especially in tissue samples with highly oriented cells and low-power, large back-aperture objectives.

 

How The Rotating Nosepiece Module Supports High-Precision, High-Contrast Multiphoton Imaging

The nosepiece allows tilting the objective at an angle. This is especially useful when the structure of interest is not aligned along the axis of the objective lens in the vertical position, as is often the case when imaging live animals. Moreover, mounting a piezo stage onto the nosepiece allows for high-speed volumetric imaging off-axis to capture the dynamics of cellular activity.

The rotating nosepiece module for multiphoton microscopes is available in both manual and motorized configurations. Users can control the motorized nosepiece through software supplemented by an external controller secured close to the microscope. This allows for visual control of the objective’s placement in relation to the sample. The module is also supplied with exchangeable optics to suit experimental needs.