In this video, a red blood cell is pinned down to the petri dish floor by two traps while a third one is used to stretch the cell membrane.
With the 'Multiplexing' feature of the NanoTracker optical tweezers platform, three time-shared traps are generated. While two of the time shared traps pin down the red blood cell onto the surface, a third trap with an amine-modified bead in it pulls at the cell membrane. In this way an external stress is applied onto the membrane. All of the deformation is done without destroying the cell.
In this video, a yeast cell is directly trapped and with controlled speed and direction is brought in a contact with a glass wall. This is a model experiment of using NanoTracker optical tweezers in implant materials research, biofouling etc.
10 µm bead was used to perform a elasticity map on CHO cells. Imaged with the CellHesion 200 AFM.
Cell elasticity measurements on fibroblast cells. Hertz fit was used to determine a Young´s Modulus of 2kPa. Imaged with the CellHesion 200 AFM.
Adhesion measurement of cell-cell interaction between MDCK cells. Imaged with the CellHesion 200 AFM.
CHO cell bound to the cantilever with ConA, collagen-coated surface. Imaged with the CellHesion 200 AFM.
Force curve of a melanoma cell on the cantilever adhering to an endothelial cell on the surface. Imaged with the CellHesion 200 AFM.