In this video, a red blood cell is pinned down to the petri dish floor by two traps while a third one is used to stretch the cell membrane.
With the 'Multiplexing' feature of the NanoTracker optical tweezers platform, three time-shared traps are generated. While two of the time shared traps pin down the red blood cell onto the surface, a third trap with an amine-modified bead in it pulls at the cell membrane. In this way an external stress is applied onto the membrane. All of the deformation is done without destroying the cell.
In this video, a yeast cell is directly trapped and with controlled speed and direction is brought in a contact with a glass wall. This is a model experiment of using NanoTracker optical tweezers in implant materials research, biofouling etc.
CHO cell bound to the cantilever with ConA, collagen-coated surface. Imaged with the CellHesion 200 AFM.