KEYWORDS: Optical Tweezers; NanoTracker; Fluorescence Microscopy; Optical Integration; Optical Trap; Force Measurements; DNA; DNA Bridges; Nanomanipulation
The NanoTracker Optical Tweezers (OT) setup delivers the ground-breaking capabilities researchers need to unravel fundamental processes taking place in living cells. This system enables the non-invasive trapping and manipulation of biological samples in both in vitro and in vivo studies. It is a powerful research tool for investigating real-time interactions in living cells, single molecules, viruses, and bacteria with nanometer precision and femtonewton resolution.
This application note outlines how optical tweezers can be successfully applied to study DNA damage, repair mechanisms, and protein-mediated DNA bridges. We demonstrate that interaction forces between individual molecules, the strength of the intermolecular connection, and the rupture force necessary to break protein-mediated DNA bridges apart can be precisely measured and quantified.