Optical Tweezers

NanoTracker™ 2

Modular force-sensing optical tweezers and optical trapping platform
NanoTracker 2

Highlights

NanoTracker™ 2

The NanoTracker™ 2 is an optical tweezers platform based on research-grade inverted optical microscopes and designed for sensitive manipulation, force and tracking experiments. With the NanoTracker™ 2, the user can trap and track particles from several µm down to 30nm with the ability to control, manipulate and observe samples in real time with nanometer precision and femtoNewton resolution.

Multiple Traps
Precise optical trapping and 3D-manipulation
Trap and track particles from several µm to 30 nm: Beads, oil bubbles, bacteria, single-molecules, and small cells. For live cells studies and materials science.
Smallest Forces
Precise force measurements
Quantitative sub-pN force measurements and position tracking at MHz sampling rates. Ultra-stable 1064 nm trapping laser.
Versatile
Couple with confocal microscopy
Combine with standard optical microscopies, AFM and Raman-spectroscopy. Simultaneous optical trapping, tracking, and advanced fluorescence investigation.

Features

Features

NanoTracker™ 2 - designed for quantitative force measurements

With the NanoTracker™ 2, the user can trap and track particles from several µm down to 30nm with the ability to control, manipulate and observe samples in real time with nanometer precision and femtoNewton resolution.

NanoTracker™ technology provides precisely quantifiable and reproducible measurements of particle/cell interactions. The system delivers precise information about single molecule mechanics and may also be used to determine mechanical characteristics such as adhesion, elasticity or stiffness on single molecules.


The video shows confocal scanning microscopy combined with optical particle manipulation

Highest performance & modular design

The new system is designed to detect the smallest forces and manipulate particles or molecules with the highest precision. Special laser stabilization and newly designed detection electronics in the head provide to very low noise levels. Additionally, the compact folded design of the laser beam path makes the system immune to drift.

Double-beam or multi-beam configurations, combined solutions for coarse and extra precise sample positioning give the user flexibility. Several beam steering options including the newly designed pivot-point piezo-driven mirrors and fast acousto-optic deflectors (AODs) perfectly match requirements of any application.

In addition to extensive sample positioning control including a customized closed-loop piezo sample stage option, the traps can be steered individually in 3D through the sample. Moreover, the laser power can be controlled for both traps independently. This freedom is required to allow a wide range of experimental assays and geometries.

The two traps are available full time and are generated from a single laser source by polarization splitting. This makes the system ultra-stable against drift.

The new back focal plane interferometry detection unit of the NanoTracker™ 2 is equipped with individual detectors for each trap having separate diodes to lateral (XY) and axial (Z) displacements of the trapped bead.

Such a detection approach, in combination with software-controlled dimming filters, allows the use of the full dynamic range of the detectors, achieving the highest possible sensitivity for any selected bead types, laser intensities and trap split ratios.

Important for exact force measurements are precise calibration of the traps, lowest position noise and a flat trap stiffness profile over a large field of view. The new precise and flexible one-button trap calibration procedure is independent from bead size and medium viscosity. The cross-talk between trap signals in the detection is drastically reduced.

Single-molecules & biopolymers

DNA elasticity measurement [1] and ds-DNA stretching between two trapped beads in force-clamping mode [2]
  • Intra-molecular elasticity & protein folding dynamics
  • Motor protein tracking
  • DNA/RNA mechanics
  • Protein-DNA binding
  • Nanopores & 3D polymer network probing

Cell-particle interaction and infection studies

  • Membrane organization (e.g., lipid rafts)
  • Trans-membrane processes, trafficking
  • Intracellular forces
  • Receptor-ligand experiments
  • Cell mechanics and cell motility
  • Membrane tether dynamics
  • Micro-rheology of cells and gels
The images show the JPK PetriDishHeater™ for live-cell experiments, CHO cell with a membrane pulled by an optically trapped protein-coated bead [1] and corresponding force vs. distance plot [2]

Cell-particle interaction and infection studies

Bright field image of a MDCK cell approaching and retracting a carboxyl-coated polystyrene bead [1] and corresponding force vs. distance plot [2]. Single-virus force measurement: Influenza virus-coated beads where moved toward a cell until touching, and subsequently retracted [3] (Adopted from C. Sieben et al., PNAS 2012, vol. 109 pp. 13626-31.).
  • Tracking of pathogen-host interaction and escape forces
  • Bacterial and virus adhesion forces
  • Local gene or drug delivery
  • Entrance mechanism studies
  • Nanotoxicity & endocytosis studies

Advanced measurements

Bright field image of four polystyrene 2 μm beads held by multiplexed traps [1] and force measurements obtained during viscous drag experiment, where a piezo was oscillating with constant speed of 100 μm/s [2]. Thermal motion plot of a 1 µm silica particle in the trap volume [3].
  • Complex optical trap geometries
  • Optical guiding & artificial crystal building
  • Local field enhancement & Raman/SERS applications
  • Brownian motion tracking, Photonic Force Microscopy (PFM)
  • Colloidal and polymer meshworks force probing
  • Video particle tracking and optical spectroscopy

Key Features

  • 3D force measurements with femto-Newton sensitivity and sub-nm precision
  • Highest stability and lowest noise level for the most accurate measurements
  • Simultaneous fluorescence imaging
  • Powerful, flexible control and data analysis software
  • Class 1 laser certified
  • Flexible, modular design for applications ranging from single-molecules to living cells

Applications

Applications

NanoTracker Data Gallery

Bruker’s BioAFMs allow life science and biophysics researchers to further their investigations in the fields of cell mechanics and adhesion, mechanobiology, cell-cell and cell-surface interactions, cell dynamics, and cell morphology. We have collected a gallery of images demonstrating a few of these applications.

Specifications

Specifications

Operating Modes

Instrument modes

  • 3D particle tracking
  • Point and Trap™ for easy trap positioning
  • Online calibration based on power spectrum
  • Advanced Force Spectroscopy including Force Clamp and Force Ramp with the new JPK RampDesigner™
  • Active and passive force mapping
  • Micro-rheology mode
  • Optical sorting
  • Trap oscillation
  • Multiplexing for 3D trap arrays
  • Line traps & circular traps
  • Microfluidics control
  • Nanoassembly
  • Optical z-stacking

Advanced optical microscopy modes

  • Brightfield transmission illumination (standard)
  • Differential Interference Contrast (DIC) in transmission (standard)
  • Epi-fluorescence microscopy (standard)
  • Raman spectroscopy
  • TIRF microscopy
  • Confocal microscopy
  • FRET microscopy and more

 

 

Accessories

Accessories

The Widest Range of Accessories in the Market

Optical systems/accessories, electrochemistry solutions, electrical sample characterization, environmental control options, software modules, temperature control, acoustic and vibration isolation solutions and more. Bruker provides you with the right accessories to control your sample conditions and to perform successful experiments.  

Webinars

Webinars

Watch Recent BioAFM Webinars

Our webinars cover best practices, introduce new products, provide quick solutions to tricky questions, and offer ideas for new applications, modes, or techniques.

More Information

Contact Expert

Contact Us

* Please fill out the mandatory fields.

Please enter your first name
Please enter your last name
Please enter your e-mail address
Please enter your Company/Institution
What best describes your current interest?
Please add me to your email subscription list so I can receive webinar invitations, product announcements and events near me.
Please accept the Terms and Conditions

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.