NanoTracker 2
The NanoTracker 2 Optical Tweezers system enables the trapping and manipulation of microscopic objects on the nanoscale, and the exertion and measurement of piconewton-scale forces.
The unrivalled sensitivity and stability of NanoTracker 2 allows the investigation of nanoparticles and quantification of their interactions in materials science, as well as the study of samples ranging from molecules, proteins, and sub-cellular components to bacteria, emulsions, and polymers in the life sciences.
NanoTracker 2-为定量力学测试而生
通过NanoTracker 2，用户可以捕获和追踪从几微米到30纳米的颗粒，并能以纳米级的精度和飞牛级的分辨率实时控制、操纵和观察样品。
NanoTracker技术可对颗粒/细胞的相互作用进行精确定量和可重复的测量。该系统可提供单分子力学的精确信息，也可用于检测单分子的机械特性，如粘附力、弹性或刚度。
该视频显示了共焦扫描显微镜与光学粒子操作相结合的情况
最好的表现以及模块化设计
这个新系统用于检测最小的力并以最高精度操纵粒子或分子。扫描头中的特殊激光稳定装置和新设计的检测电子装置保证了非常低的噪音水平。此外，激光束路径的紧凑折叠设计使该系统不受漂移的影响。
双光束或多光束的配置，粗略地和特别精确地样品定位的组合解决方案，为用户带来了灵活性。多种光束转向选项，包括新设计的枢轴点压电驱动镜和快速声光偏转器（AODs），完美地满足任何应用的要求。
除了包含一个定制的闭环压电样品台选项的样品定位控制，捕集器也可以在三维中单独操纵样品。此外，两个捕集器的激光功率可以独立控制。这种自由度满足宽泛的实验测定和几何形状的要求。
这两个捕集器是通过偏振分裂从一个激光源产生，并且全时可用。这使得该系统具有超稳定的抗漂移能力。
NanoTracker 2的新后焦平面干涉检测单元为每个捕集器配备了单独的检测器，由独立的二极管来检测捕集bead的横向（XY）和轴向（Z）位移。
这种检测方法与软件控制的调光过滤器结合，允许检测器在全部动态范围使用，对于任何选定的beads类型、激光强度和捕集器分裂比都能实现最高的灵敏度。
对于精准的力测量来说，重要的是精准的捕集器校准，最低的位置噪声和大视野下平坦的捕集器硬度曲线。新的精准而灵活的一键式捕集器校准程序不受bead大小和介质粘度的影响。检测中捕集器信号之间的交叉干扰大大减少了。
单分子和生物高分子
· 分子内弹性与蛋白质折叠动力学
· 机动蛋白追踪
· DNA/RNA机械学
· 蛋白质-DNA结合
· 纳米孔和三维聚合物网络探测
细胞与颗粒的相互作用和感染研究
· 膜组织（如脂质筏）
· 跨膜过程，贩运
· 细胞内力
· 受体-配体实验
· 细胞力学和细胞运动
· 膜拴的动态变化
· 细胞和凝胶的微流变学
细胞与颗粒的相互作用和感染研究
· 跟踪病原体与宿主的互动和逃逸力
· 细菌和病毒的粘附力
· 局部基因或药物输送
· 入口机制研究
· 纳米毒性和内吞作用研究
先进测试
· 复杂的光学陷阱几何形状
· 光学引导和人工晶体的建立
· 局部场增强和拉曼/SERS应用
· 布朗运动追踪，光子力显微镜（PFM）
· 胶体和聚合物网状结构的力探测
· 视频级粒子跟踪和光学光谱学
Precise Trap Calibration
Accurate force measurements require precise trap calibration.
NanoTracker 2 delivers:
- One-click trap calibration
- Lowest position noise and consistent trap stiffness over a large field of view
Cutting-Edge Electronics
At the center of the NanoTracker 2’s stability, nanometer-precise position accuracy, and lowest noise levels is the high-performance electronics control unit.
External equipment, such as advanced cameras, spectrometers, and detectors (e.g., PMTs or APDs), can be integrated or triggered using TTL signals.
The high-bandwidth controller ensures real-time data acquisition, feedback, and response - essential for force clamp experiments and sensitive control of particle motion.
Intuitive Software
The straightforward, flexible, and userfriendly software of the NanoTracker 2 provides:
- Fast, intuitive control of all motorized components, e.g., objectives, sample stage, and syringe pumps
- Scriptable software interface for automated experiment workflows. Suitable for beginners and experienced users alike
- Powerful DataProcessing software with models for single-molecule force spectroscopy (worm-like chain, freely jointed chain, etc.) and stepfitting routines
Safe by Design
NanoTracker 2 is a certified Class 1 laser product and suitable for multidisciplinary environments. A designated laser laboratory is not required.
- No specific laser safety training necessary
- Suitable for biological experiments: All components are protected from liquids
Advanced Functionality
A wide range of add-ons, accessories, experimental modes, and features greatly extend the scope of applications possible. These include:
Point and Trap functionality:
a “live” optical image is seamlessly integrated for easy control of trap positions
Absolute Force Spectroscopy:
performs force-distance experiments by moving the trap or sample at user specified speed and direction
Ramp Designer:
for customized force spectroscopy experiments, such as force ramp and force clamp
CalibrationManager:
one-click calibration of trap stiffness and position detection system
ExperimentPlanner module:
easy design of customized experiment routines and automation scripts
Precise calibration of optical image:
enables highly precise positioning of trap
LaminarFlowCell (LFC) for Single-Molecule Applications
The multichannel LFC is a coverslip-based fluid cell with laminar flow perfusion capabilities. It is ideal for multi-component samples or multistep experiments. Features include:
- Up to five separate input channels, one output channel, and variable channel heights
- Software-controlled syringe pumps for automated fluid flow and exchange
- Sample heating up to 45 °C
Combination with AFM (OT-AFM)
NanoTracker 2 can be seamlessly combined with Bruker’s NanoWizard AFM platform via the OT-AFM ConnectorStage.
This setup combines the 3D positioning and manipulation capabilities of optical tweezers with the high-resolution imaging, force detection, and surface property characterization capabilities of AFM, opening an entirely new spectrum of applications:
- Enables the measurement of forces in the range of 500 fN to 1mN on the same sample
- Combine OT-AFM with optical techniques, such as TIRF and confocal microcopy, for comprehensive samples analyses
Advanced Applications
Live Cell Applications
Advanced environmental control options allow the investigation of living cells under near-physiological conditions:
- Study the mechanisms of infection, immune response, and bacterial/virus/nanoparticle uptake processes. Quantify bacteria-cell or virus-cell adhesion and interaction forces
- Perform membrane tether pulling and cell indentation experiments
- Quantify the mechanical properties of living cells, their adhesion forces, and dynamic mechanisms
- Investigate protein-ligand binding events
- Trigger cellular response using functionalized particles or modified micro-organisms
- Study cell-cell, cell-surface, or cell-matrix interactions
Cell Manipulation
As well as applying and measuring forces, NanoTracker 2 can be used to manipulate cells directly with optical traps:
- Sort, deform, relocate, or stretch cells
- Manipulate particles or organelles inside living cells
Microrheology Applications
NanoTracker 2 enables the investigation of the microrheological properties of a broad range of samples, such as cells, soft matter, or gels.
- Quantify the viscoelastic and mechanical behavior of soft materials, biological matter, and emulsions
- Perform non-destructive, highly sensitive measurements in delicate systems
- Collection of bead movement spectra at up to 7.5 MHz
- High power laser option (up to 10W) for advanced viscoelastic measurements in highly viscous environments
Single-Molecule Mechanics
The high spatial, temporal, and force resolutions of NanoTracker 2 provide the precision and stability necessary to investigate single-molecule mechanics and intramolecular forces.
Perform manipulation experiments at sub-nm, sub-pN, and μs resolution, while executing reproducible, standardized experiments.
3D Tracking
Passive and active 3D tracking (also called force- or position-clamping) are two of the main tools used to investigate processivity and force generation in motor proteins, cell membrane trafficking, binding events, and DNA-polymerase interactions.
The force-clamping feature of NanoTracker 2 applies a force to the sample that is kept constant via the feedback system. This allows researchers to study the response of a molecule to a specific force - useful in the investigation of unfolding or refolding events.
主要特点
· 具有飞牛灵敏度和亚纳米精度的三维力测量
· 最高的稳定性和最低的噪音水平，实现最精确的测量
· 同时进行荧光成像
· 强大、灵活的控制和数据分析软件
· 一级激光认证
· 灵活的模块化设计，应用范围从单分子到活细胞
NanoTracker 数据展
布鲁克公司的BioAFM使生命科学和生物物理学研究人员能够在细胞力学和粘附、力学生物学、细胞-细胞和细胞-表面相互作用、细胞动力学和细胞形态学等领域进一步开展研究。我们收集了一些展示其中一些应用的图片。
操作模式
· 三维粒子跟踪
· 点式和捕获式，便于捕获定位
· 基于功率谱的在线校准
· 先进的力谱分析，包括使用新的JPK RampDesigner的力钳和力斜坡。
· 主动和被动力谱成图
· 显微流变学模式
· 光学分选
· 陷波器震荡
· 三维陷波器阵列的复用
· 线形捕集器和圆形捕集器
· 微流变控制
· 纳米组装
· 光学Z型堆叠
先进的光学显微镜模式
- 明视场透射照明（标准）
- 透射式差分干涉对比（DIC）（标准）
- 外延式荧光显微镜（标准）
- 拉曼光谱学
- TIRF显微镜
- 共聚焦显微镜
- FRET显微镜及更多
市场上种类最多的配件
光学系统/配件、电化学解决方案、电学样品表征、环境控制选项、软件模块、温度控制、声学和振动隔离解决方案等。布鲁克公司为您提供合适的配件，以控制您的样品条件并开展成功的实验。
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