Fluorescence Microscopy Webinars

Imaging Hippocampal Microcircuitry Using Glass Microperiscopes

Learn how to image the canonical hippocampal microcircuit using two-photon microscopy.

Understanding how the hippocampal microcircuit contributes to behavior

During this live event, Dr. Goard  will demonstrate a new approach for two-photon imaging of the transverse plane of the murine hippocampus using chronically implanted glass microperiscopes, allowing us to measure dendritic morphology and functional activity during behavior.

Tuesday, November 29 — 10AM PST | 1PM EST | 7PM GMT+1


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Presenter's Abstract

The hippocampus consists of repeated motifs of a canonical neuronal microcircuit first described by neuroanatomists over a century ago. This microcircuit contains distinct subfields (CA1-3, DG) with stereotyped connectivity that are critical for memory formation. However, optical imaging of the canonical circuit has not been possible in intact animals. To address this, our lab developed an approach for two-photon imaging of the transverse hippocampal plane in awake mice via implanted glass microperiscopes. This approach allows us to track dendritic morphology over repeated sessions and measure functional responses (e.g., place fields) using genetically encoded calcium indicators. In this talk, I will describe the procedure, imaging results, and discuss general considerations for the use of glass microprisms for deep brain imaging.

Image of the transverse plane of the intact hippocampus through an implanted microperiscope in a transgenic with sparse green fluorescence expression in excitatory neurons (Thy1-GFP-M line)


Michael Goard, Ph.D., Associate Professor, Department of Molecular, Cellular and Developmental Biology, Department of Psychological & Brain Sciences

Michael Goard received his B.A. in Psychology from Reed College and his Ph.D. in Neuroscience from UC Berkeley, where he completed his dissertation in the lab of Prof Yang Dan. As a postdoctoral fellow with Prof Mriganka Sur at MIT, he developed approaches for imaging the activity of large populations of neurons in behaving mice. He joined the faculty of UC Santa Barbara in 2016, where his lab investigates the neural circuitry underlying our perceptual, spatial, and cognitive abilities.