Exploring in vivo Neural Circuit Activity Webinar Banner

Exploring in vivo Neural Circuit Activity with Simultaneous All Optical Manipulation and Recording

Multiphoton microscopy has for several years provided a useful tool for imaging the activity of neural networks in vivo at the cellular level, as well as providing an optogenetic tool for optically stimulating individual neurons.
This webinar took place on March 25th 2015

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In this webinar, Dr. Packer reviews his recent work in which he utilized a Spatial Light Modulator (SLM) interfaced to a multiphoton microscopy system in order to simultaneously use optical stimulation and optical recording to study neural networks. A major benefit of the use of the SLM is that tens of neurons can be simultaneously stimulated, while at the same time imaging neuronal activity in a field using a calcium indicator.

Dr. Packer explores principles of the use of SLMs for the study of neural networks, including data demonstrating the validity of the approach. He also discusses how the use of SLMs as part of multiphoton microscope systems will impact future research on neural networks in vivo in awake behaving animal paradigms.


Dr. Adam Packer
Dr. Adam Packer
Wellcome Trust Sir Henry Dale Fellow, University of Oxford
Dr. Packer has over 15 years of experience designing, building, and optimising multiphoton microscopes to expand their capabilities, with specific expertise in optogenetics, calcium imaging, multi-cell targeted photostimulation, digital holography, and software development. He studied neuroscience and biomedical engineering at MIT before completing a PhD at Columbia University with Dr. Rafael Yuste. As a Marie Curie Fellow at University College London in Dr. Michael Häusser’s lab, Adam developed a new approach using two-photon imaging and optogenetics to both readout and manipulate neural circuit activity in vivo. He has a strong interest in technology dissemination and accessibility, with a track record of combining experimental, computational, and theoretical approaches to design experiments and analyse data to understand structure and function in neural circuits. His research group is based at University of Oxford and uses all-optical interrogation techniques he has helped pioneer to investigate neural coding principles in behaving animals.