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Light as the Independent Variable: Photomanipulation Techniques for Confocal and Multiphoton Microscopy

A variety of techniques are available to biological microscopists that allow them to use light as a stimulus to produce measurable changes in their sample of interest. These techniques include uncaging, fluorescence recovery after photobleaching (FRAP), photoactivation, photoconversion and photoablation.
This webinar took place on June 18th 2015

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Overview

In this webinar, we explore the basic principles of these various techniques and examine their value in various experimental protocols. Examples of the various techniques will be presented using data collected on Bruker Ultima multiphoton microscopes and Opterra multipoint scanning confocal microscopes.