heliX⁺

• 2 detection spots x 2 colors
  for real-time referenced measurements of 2 interactions, or monitoring of up to 4 interactions simultaneously.
• automated sensor exchange
  load up to 5 chips in one rack and switch automatically between 5 channels and 10 sensor spots.
• NFC near field communication
  between instrument and chip for seamless traceability of chip status and usage history. Ideal for consistent, error-free results. 
• advanced microfluidics
  minimalist single-channel design, engineered for robustness: made from durable glass, withstanding highest flow rates and corrosive chemicals, disposable.
• Temperature Control
  Sample temperature adjustable between 15°C and 40°C.

Combine fluorescence sensing with time-resolved single-photon counting technology to get superior performance that is applicable to almost any molecular experiment.

• Epi-fluorescence set-up with high-numerical aperture for optimal photon collection.
• Simultaneous detection of four signals (2 x 2) by four single-photon detectors.
• Two-color detection, red and green, from each sensor spot.
• Two detection spots in each flow channel for real-time referencing of two colors or multiplexing of four interactions.
• Proprietary E-TCSPC electronics (Electrically-triggered Time Correlated Single Photon Counting) for nanolever switching speed measurements with 0.1 µs resolution.

Measurement modes

• Detection of molecular binding via fluorescence proximity sensing.
• Analysis of molecular friction (conformational) changes by E-TCSPC nanolever switching speed measurements.
• Analysis of binding, ternary complexes and nucleic acid conformational changes via two-color FRET

heliX^® microfluidics were designed from scratch, based on many years of experience with binding kinetics measurements in flow.

The cross-over design with integrated microvalves enables the flow of analyte and buffer from two opposite directions across the sensor. 

This allows for a rapid solution exchange, unrivalled by any other commercially available system. Fluidics can be operated using classical one-way flows, too. Every component has been optimized for fast response times and minimal sample consumption, while ensuring maximal robustness for years of maintenance-free operation.

• rapid flow switching within 20 ms
• no cross-contamination
• maintenance-free (no IFC)
• in-run sample line washing

• Automated workflows.
• Fully automated throughput using 96 or 384 well plates.
• Additional space for different glass vials.
• Temperature controlled sample compartment (4 – 40 °C).

• Control of several heliX^® units at the same time for scalable throughput
• modular connection to heliOS network
  Autonomous device operation with embedded control system.
  Stable data transfer to control PC.