heliXcyto
Kinetics on Cells
Biosensor meets Cytometry
Automated real-time binding measurements directly on cells
The heliXcyto chip
heliXcyto chips feature flow-permeable, bio-compatible polymer cages, which physically retain any type of cells.
Automatic exchange of 5 chips is possible and chips are equipped with RFID tags for seamless traceability.
The sample temperature during measurement can be adjusted between 15°C and 40°C.
Cells are trapped automatically from a stock solution within seconds
Chip types and sizes
heliXcyto chips enable single- and multi-cell measurements. Trap sizes are tailored to capture single cells and chip options support cell sizes between approx. 6 – 25 µm.
heliXcyto optical detection system
- Cell imaging with built-in reflected light microscope.
- Two-color detection, red and green, for monitoring two processes.
- Ultra-sensitive fluorescence detection for analysis of low membrane protein expression levels.
- Second spot for real-time referencing.
heliXcyto advanced fluidics system
- High performance microfluidics for resolving fast and slow kinetics.
- Flow rates up to 500 µL/min for artefact-free measurements of rapid kinetics.
- Compatible with complex media.
- Reverse flow for chip regeneration.
heliXcyto autosampler
- Automated workflows.
- Fully automated throughput using 96 well plates.
- Temperature controlled sample compartment (4 – 40 °C).
- Automated cell resuspension before sample uptake.
heliXcyto automation and scalable throughput
- Control of several heliX® units at the same time for scalable throughput
- modular connection to heliOS network
Autonomous device operation with embedded control system.
Stable data transfer to control PC.
heliXcyto Applications
The heliX® biosensor utilizes single-cell Interaction Cytometry technology to measure kinetics in real time directly on cells:
- association kinetics (kon)
- dissociation kinetics / half-life (koff, t1/2)
- affinities and avidities (Kd)
"Until recently I always said, unfortunately we cannot do that on cells, kon and koff. A great answer now is with the heliXcyto we can. I like that a lot. Because this is really new and gives us new information."
Prof. Harald Kolmar, Professor, Kolmar Group, TU Darmstadt, Darmstadt, Germany
