heliX cyto

unique design features

Automated real-time binding measurements directly on cells

Native environment

Measure association and dissociation kinetics, half-life, affinity and avidity directly on cells, preserving the native folding, density, mobility and co-interactions of your target.

Unique cell traps

We use unique cell traps to physically retain the cells on the chip surface. There is no need for any modifications or specific receptors on the cells’ surface.

Any cell type

Trap any eukaryotic cell type you want, adherent or suspension, fixed or alive, cell line or primary cells.

Automation

From cell culture to interaction data in three simple steps with less than 1 h hands-on time thanks to automated workflows.

The heliX^cyto chip

heliX^cyto chips feature flow-permeable, bio-compatible polymer cages, which physically retain any type of cells.

Automatic exchange of 5 chips is possible and chips are equipped with RFID tags for seamless traceability.

The sample temperature during measurement can be adjusted between 15°C and 40°C.

Cells are trapped automatically from a stock solution within seconds

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Chip types and sizes

heliX^cyto chips enable single- and multi-cell measurements. Trap sizes are tailored to capture single cells and chip options support cell sizes between approx. 6 – 25 µm.

heliX^cytooptical detection system

Cell imaging with built-in reflected light microscope.
Two-color detection, red and green, for monitoring two processes.
Ultra-sensitive fluorescence detection for analysis of low membrane protein expression levels.
Second spot for real-time referencing.

heliX^cytoadvanced fluidics system

High performance microfluidics for resolving fast and slow kinetics.
Flow rates up to 500 µL/min for artefact-free measurements of rapid kinetics.
Compatible with complex media.
Reverse flow for chip regeneration.

heliX^cyto autosampler

Automated workflows.
Fully automated throughput using 96 well plates.
Temperature controlled sample compartment (4 – 40 °C).
Automated cell resuspension before sample uptake.

heliX^cytoautomation and scalable throughput

Control of several heliX^® units at the same time for scalable throughput
modular connection to heliOS network
Autonomous device operation with embedded control system.
Stable data transfer to control PC.

heliX^cyto Applications

The heliX^® biosensor utilizes single-cell Interaction Cytometry technology to measure kinetics in real time directly on cells:

association kinetics (k_on)
dissociation kinetics / half-life (k_off, t_1/2)
affinities and avidities (K_d)

Find out more about the single-cell interaction cytometry technology and the different applications.

"Until recently I always said, unfortunately we cannot do that on cells, k_on and k_off. A great answer now is with the heliX^cyto we can. I like that a lot. Because this is really new and gives us new information."

Prof. Harald Kolmar, Professor, Kolmar Group, TU Darmstadt, Darmstadt, Germany