Exploring 2D HSQC NMR
Discover the latest advancements in 2D HSQC NMR with this comprehensive application note, featuring Bruker parameter sets and showcasing innovative pulse sequence variants to enhance your HSQC applications.
The application Note presents a comprehensive overview of two-dimensional Heteronuclear Single Quantum Coherence (2D HSQC) NMR spectroscopy, focusing on the use of Bruker parameter sets to optimize structural elucidation of unknown compounds. It details the evolution of the HSQC pulse sequence, highlighting improvements in sensitivity, resolution, efficiency, and robustness. The guide is designed to assist both routine and advanced users in selecting and applying the most suitable HSQC methodologies for their analytical needs, supporting reliable structure verification, accelerated data acquisition, and quantitative analysis in chemistry and life sciences.
Key points
- Comprehensive coverage of HSQC NMR variants, including conventional, sensitivity-enhanced, multiplicity-edited, COSY-suppressed, adiabatic decoupling, non-uniform sampling, ultrafast, pure shift, CLIP, and perfect HSQC, with corresponding Bruker parameter sets and setup instructions.
- Practical optimization tips for experiment setup, parameter selection, and data acquisition, illustrated with real-world spectra and workflow guidance in Bruker TopSpin.
- Advanced methodologies for quantitative and high-resolution NMR analysis, enabling reliable structure verification, coupling constant measurement, and robust sample characterization.