InsightCell for High-Field

InsightCell™ is based on the established InsightMR™ platform, bringing Bruker’s trusted expertise in flow NMR to the study of living cells. It is designed for real-time on-line metabolite monitoring and enables accurate quantification of dynamic metabolic changes. InsightCell offers a powerful window into cellular function while keeping your bioprocess running continuously.

• Continuous Bioprocess Monitoring: Whether feeding cells with fresh media inside the flow tube or transferring culture fluids directly from bioreactors, InsightCell tracks metabolic changes as they happen.
• Fermentation Optimization: Monitor key metabolites like ethanol or lactate in real time. Use this data to fine-tune pH and temperature, improving efficiency and reproducibility.
• Versatility Across Applications: From antibiotics and vaccine production to vitamin synthesis and enzyme engineering, InsightCell empowers you to control and optimize critical bioprocess parameters.

InsightCell

• Biocompatible flow cell for live-cell cultures
• Low-shear design preserves cell viability
• Temperature-controlled transfer lines: -20 to 120 °C capability
• Compatible with standard high-field NMR probes
• Compatible with all Bruker sample changers
• InsightMR 2.0 software  for automation and analysis

InsightMR Software 2.0

InsightMR is a software platform for real-time analysis of chemical and bioprocesses using on-line and in-line NMR. It allows on-the-fly adjustment of acquisition parameters, supports a wide range of experiments, and handles large series of spectra efficiently. Data are seamlessly transferred to Mnova for further analysis, with results delivered as ready-to-use kinetic and waterfall plots.

• Real-time metabolite profiling without sampling interruptions
• Continuous media exchange during NMR acquisition
• Direct connection to bioreactors and perfusion systems
• Straightforward installation and operation with minimal learning curve

Online monitoring of living cells metabolism by nuclear magnetic resonance:

https://www.bioaster.org/fr/un-projet-avec-bioaster/study-cases/insightcell/

Intra- and extracellular real-time analysis of perfused fibroblasts using an NMR bioreactor: A pilot study

Metabolomic discrimination of various mitochondrial defects presents a significant challenge. This study describes an NMR-based bioreactor system that enables real-time investigation of intra- and extracellular metabolism in perfused fibroblasts.

https://doi.org/10.1002/jimd.12794

Determination of Intra- and Extracellular Metabolic Adaptations of 3D Cell Cultures upon Challenges in Real-Time by NMR

This study presents a perfused NMR bioreactor system enabling real-time, quantitative analysis of intra- and extracellular metabolites in human fibroblasts. Using diffusion NMR, 35 metabolites were detected, with 19 successfully separated by compartment. The approach demonstrates sensitivity to metabolic changes under different media and flow rates.

https://doi.org/10.3390/ijms23126555

Live monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR spectroscopy

This study presents a reproducible NMR bioreactor system for simultaneous 1H-NMR metabolite profiling and 19F-NMR oxygenation monitoring in 3D collagen-based cell cultures. A standardized setup enables dynamic assessment of mitochondrial respiration and metabolic flux under perfusion

https://doi.org/10.1039/D1AN00041A

Real-time Metabolic Investigations of Living Cells by NMR – First Experiences

This study demonstrates the feasibility of real-time metabolic monitoring in 3D cell cultures using the commercially available InsightCell™ tool, enabling reproducible glucose and oxygen measurements over 12 hours. Metabolic interventions triggered rapid, reversible shifts between aerobic respiration and anaerobic glycolysis.

https://cds.ismrm.org/protected/19MProceedings/PDFfiles/4375.html