Training

Introduction to TopSpin for Solid-State NMR

Introduction to TopSpin software for operation of Bruker AVANCE NMR Spectrometers with solid-state capabilities

Introduction To TopSpin For Solid State NMR

This 4 day course is designed to introduce users to the Topspin software, provide a greater understanding of the many features within the software, and practical experience at the spectrometer setting up basic Solid State NMR experiments for spin ½ nuclei.

The course will be taught focusing on TopSpin 4 software, but the majority of concepts are backwards compatible to older versions of TopSpin.

Lectures will be followed by a laboratory session in front of a spectrometer for hands on experience.

The lecture portion of the course is done in conjunction with the liquids Introduction to TopSpin

Topics and Experiments:

Basic sample preparation and rotor packing
Setting up a CPMAS probe:
- Setting magic angle
- Pulse calibration
- Shimming
- Optimizing Hartmann-Hahn match condition
Standard 1-Dimentional ¹³C CPMAS experiments and various spectral editing sequences
- Spinning sideband suppression (TOSS)
- Dipolar dephasing with and without TOSS
- CPPI for signal assignment
Setup CPMAS for different Nuclei (¹⁵N ²⁹SI or ³¹P)
2-Dimentional structure elucidation experiments (FSLG Hetcor)
Processing (widow function, FT, phasing, baseline correction)
Post Processing (peak picking, integration)
Presenting the data (saving/plotting spectra)
Quantitative NMR (qNMR)
Introduction to automation
Computer / TopSpin administration
Variable Temperature