Training

NMR Methods for Labeled Proteins

Overview

Topics:

  • Overview of NMR experiments for protein structure determination.
  • Introduction to triple-resonance experiments.
  • Bruker software for biomolecular applications.
  • Set-up and acquisition of triple-resonance experiments.
  • Introduction to shaped pulses.
  • Recent methodological developments in bio-NMR


Remarks:


This is a course for experienced users of Avance spectrometers working with labelled protein samples. The background for choosing and setting-up 3D-triple-resonance experiments for proteins is given. During the practical sessions representative spectra such as 3D-15N-NOESY-HSQC, HNHA, HNCA, HNCOCA, CBCA(CO)NH and HCCH-TOCSY are acquired. The implementation of the shaped pulses needed for biomolecular NMR and the use of adiabatic pulses, is covered.

Prerequisite Qualification:

Participation in the course Advanced NMR Methods or corresponding knowledge.

Dates:

October 7th - 8th, 2021

Location:

Further Information

Duration:

2 days

Language:

English

Costs:

€ 860 plus VAT
(incl. documentation, coffee breaks and lunch)

 

Registration

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Course Coordinator

Mrs. Svetlana Startseva
Bruker BioSpin AG