Untargeted biomarker discovery

MALDI Imaging for untargeted biomarker discovery and classification

Molecular changes originate at the cellular level and solution-based techniques lack sensitivity to capture changes produced from small cell populations.

MALDI imaging of sample cohorts along with SCiLS Lab analytical software extracts unique spatial patterns of proteins, lipids or metabolites and correlates them with sample pathology to identify and map putative biomarker ions.

breast image
Image and segmentation analysis of a breast cancer biopsy. (l to r) delete unstained optical image; average profile spectra from 4 top-most segments; PCA analysis showing distribution of top four segments; segmentation map showing spatial distribution of top-four segments based on similarity of their molecular profiles.

Analyze large sample cohorts for biomarker discovery using molecular profiles and sample metadata to find relevant biomarkers that compliment histology. SCiLS lab is the leading biomarker discovery software for analyzing mass spectrometry imaging with 2D and 3D visualization, as well as sophisticated statistical analysis with just a few mouse clicks, including: automatic spatial segmentation, co-localization analysis,  classification model calculation, ROC and component analysis (PCA,pLSA etc). 

3D kidney
3D visualization with SCiLS lab, Oetjen et al., Journal of Proteomics, 2013
Brain sections
Segmentation analysis of five serial rat brain sections

 

Bruker’s SCiLS Lab analytical software can mine hundreds of thousands of pixels using a variety of statistical tools.

Biomarker discovery protocols can accommodate fresh-frozen or formalin-fixed and paraffin embedded (FFPE) tissues or tissue microarrays (TMAs). Many tissue banks routinely fix biological specimens for long-term storage. Fixation agents act by crosslinking proteins into an insoluble Network, creating an excellent preservation technique. FFPE protocols involve additional processing steps of antigen-retrieval and protein digestion. Numerous publications have demonstrated the utiilty of imaging tryptic peptides from tissue or liberated N-linked glycans.

Hear how Bruker’s MALDI imaging tools for biomarker discovery are helping Prof. Richard Drake of the Medical University of South Carolina, to gain new insight into the glycosylation pathways of cancer development.

Literature MALDI Imaging