Cell culture

Cell Culture Media

Monitoring of nutrient elements and contaminants is an important task during production of biopharmaceuticals by cell cultures.

Total reflection X-ray fluorescence (TXRF) analysis is a rapid and cost-efficient analytical method without need for sophisticated lab infrastructure.

The use of genetically modified bacterial and mammalian cells for the production of biopharmaceuticals is growing dramatically. On the one hand, cell cultures are economically beneficial; on the other hand, certain new products like glycosylated proteins are only processed in living cells.

Cells cultivated in bioreactors require specific conditions, which are realized by cell culture media containing sugars, amino acids and a buffer system. Macro and micro nutrients play an important role and have to be fed during the production process. In addition, any (metal) contamination has to be controlled to avoid any risk of cell necrosis.

The TXRF spectrometer S4 T-STAR is a powerful tool for the control of nutrient elements and monitoring of any contaminant. Samples can be analyzed after a rapid sample preparation and the element composition is quantified without time-consuming daily calibrations. Automatic quality control procedures guarantee highest data quality during 24/7 operation.

The S4 T-STAR is a compact plug and play system without need for gases, media or advanced lab infrastructure. Operation costs are negligible and basic user skills are sufficient.

Download the full lab report XRF 468 (PDF)