rapiflex MALDI PharmaPulse

rapifleX MALDI PharmaPulse ® - Accelerate uHTS with Mass Spectrometry

The rapifleX MALDI PharmaPulse (MPP) accelerates label-free hit finding in drug discovery: rapifleX MPP combines the unambiguousness of mass spectrometry with the required speed to comb through large compound libraries of millions of substances.


The use of mass spectrometry allows the measurement of unmodified substrates in primary screens to avoid false positives or false negatives, making confirmation screens redundant. The system is designed for fully automatic handling of 1536 sample plates to screen more than a million compounds in a week in support of drug discovery.

Rapiflex MPP HighRes Biosolutions

New standard for ultra-High-Throughput Screening Label-Free ‘Hit’ Identification

By combining the speed of Bruker’s patented Smartbeam™ 3D laser technology with the direct-detection capability of mass spectrometry, the rapifleX MALDI PharmaPulse (MPP) delivers unprecedented screening capability for High-Throughput Screening biochemical assays. It allows you to screen through large and diverse compound libraries at a pace of over a million compounds a week, epitomizing the perfect balance of automation and miniaturization.

The MPP system allows for the measurement of unmodified substrates in primary biochemical screens, and offers several advantages:

  • It effectively eliminates confirmatory assays typically required of traditional fluorescence-based screening techniques
  • Saves costs for developing specific fluorescence probes or antibodies
  • Doesn’t require expensive solvents or consumables for continuous operation
  • Allow sample volume down to 25nl

 

The MPP system is specifically designed for fully automatic handling 384, 1536, 3072 and 6144 samples per plate, including automated method development and instant export to LIMS.

Durg Discovery Workflow with rapifleX MALDI Pharma Pulse

The new rapifleX MALDI time-of – flight mass spectrometer is the key enabler for the MALDI PharmaPulse solution. It represents a new era in MALDI mass spectrometry. All performance characteristics of the rapifleX MALDI TOF and TOF/TOF instrument surpasses anything seen before.

The new MALDI PharmaPulse 2.0 software solution completes the rapifleX MALDI PharmaPulse mass spectrometer with a tailored software solution for the provision of HTS campaigns – for non-experts in mass spectrometry.  This major step enables scientists working in drug discovery to use label-free mass spectrometry for large screening campaigns of millions of substances.

rapifleX MALDI PharmaPulse® - Technical Details

The all new rapifleX MALDI Time-of-Flight Mass Spectrometer is the heart of the MALDI PharmaPulse: it is the only MALDI mass spectrometer with 10 kHz scanning laser technology and measures up to 10 samples per second. All components of the system work in concert for fully automatic HTS operation. The newly designed screening software suite supports HTS workflows and works in combination with a proprietary assay development module which empowers assay developer to accelerate label-free assay development for MALDI Mass Spectrometry.

PharmaPulse Solution

The PharmaPulse Solution is based on Bruker´s advanced MALDI TOF technology and on a dedicated software package. Ease of use and time to result were key drivers during the development resulting
in an intuitive but yet powerful software solution to measure and analyze millions of samples in full automation. Two workflows guide the user from adapting an assay to MALDI mass spectrometry to
readily exported results for further analysis in LIMS systems.

method development workflow
rapifleX MALDI Pharma Pulse

Our value we bring to Drug Discovery: 

Robustness to analyze 2-3 million samples without intervention 

  • New source and ion optical design
  •  No source/ion optic cleaning within primary screens
  •  Easy & fast cleaning if required


Compatible with CyBio®-Well vario with 384 or 1536 channel simultaneous pipettor for high precision and ultra-fast sample plate preparation

Single digit cost per sample 

  • No solvent, no waste, no labeling
  • 0.025 to 1 μL sample volume
  • Low cost disposable sample plates 

Fully automatic 1536 sample plate change 

  • New ultra-fast autoloader seamlessly integrable with common robot systems
  • Rugged design tailored for uHTS operation

 

New disposable MALDI HTS sample plates 

  • Fulfills all requirements for reliable MALDI ionization
  • Suitable for 384, 1536, 3072 or 6144 geometries

rapifleX MALDI PharmaPulse® - Applications

Drug discovery workflow

A million samples per week with rapifleX MALDI PharmaPulse

The new rapifleX MPP is the result of Bruker’s innovative development of MALDI mass spectrometers. It reflects our passion for and knowledge about this technology and its unique applications.  The time-of-flight mass spectrometer (TOF-MS) of the rapifleX MALDI PharmaPulse monitors up to 30 masses in HTS mode: from small molecules to large proteins. The improved resolution and sensitivity of the rapifleX TOF-MS allows e.g. the monitoring of acetylcholine (ACh) and choline to identify acetylcholinesterase (AChE) inhibitors out of millions of substances. An ingenious detector design delivers significantly improved signal to noise ratios for enhanced sensitivity across the mass range. A completely new HTS software suite supports easy set-up of HTS screens, and the proprietary assay development module ensures fast adaption of label-free assays for MALDI mass spectrometry.

rapifleX MALDI Pharma Pulse Acetylcholinesterase
Haslam, C.; Hellicar, J.; Dunn, A.; Fuetterer, A.; Hardy, N.; Marshall, P.; Paape, R.; Pem-berton, M.; Resemann, A.; Leveridge, M. The Evolution of MALDI-TOF Mass Spectrometry toward Ultra-High-Through¬put Screening: 1536-Well Format and Beyond. J. Biomol. Screen. 2015, 1-11.

Automation of rapifleX MALDI PharmaPulse

The rapifleX MALDI PharmaPulse is designed to seamlessly integrate with laboratory automation systems to perform fully automatic screening campaigns. The MALDI PharmaPulse software solution offers an open interface to control parts of its operation from external scheduling software (e.g. Analytik Jena lab automation, HighRes Biosolutions, Beckman Coulter, Thermo Scientific automation). The new high speed autoloader of the rapifleX MPP works hand in hand with robot systems for quick loading and unloading of sample plates.

rapifleX MALDI Pharma Pulse automation

Fail Early, Fail Fast

Can the ultra-high-speed capability of the MPP to screen for hits accelerate the discovery of drug-like small molecules or biological therapeutics? Bringing a drug to market can take more than 12 years and can easily cost more than $1B dollars. Most new chemical entities fail to make it because they are either toxic or they do not work. Of the million compounds or more that are screened, 5,000 to 10,000 compounds go through the lead optimization process, resulting in about 250 compounds making it to the pre-clinical process. From this stage, about 5 make it to the critical clinical trials process, with usually only 1 compound potentially makes it to market.

 

 

rapifleX MALDI Pharma Pulse® - Service & Support

Bruker Daltonics services are performed by factory trained and certified engineers. DIN EN ISO 9001:2000 requirements are fulfilled

Available services include:

  • Help desk service for a faster access to our support team, to your service order follow up and for general information
  • Planned maintenance, according to your requirement

Available services include:

  • Help desk service for a faster access to our support team, to your service order follow up and for general information
  • Planned maintenance, according to your requirement
  • Compliance services for installation qualification, operational qualification / performance verification
  • Site planning and relocation
  • Advise to improve labs efficiency (consumable, method development & how to run the complete solution)

For Research Use Only. Not for Use in Clinical Diagnostic Procedures.