InsightMR for High-Field

High-Field NMR for PAT: Real-Time Reaction Monitoring

High-field Nuclear Magnetic Resonance (NMR) serves as an effective Process Analytical Technology (PAT) tool for real-time monitoring, optimization, and control of chemical and bioprocesses. Ideal for both industrial and academic settings, InsightMR empowers scientists to study, optimize, and control reactions with real time, on-line and in-line NMR monitoring under true process conditions. Combining a robust flow unit with dedicated software, InsightMR enables seamless integration into PAT frameworks.

Gathering insights into the mechanism and kinetics of chemical reactions is now at your fingertips!

• Provides answers to key chemical questions: reaction yield, mechanistic insights and reaction kinetics
• Harness NMR data to make strategic process chemistry decisions, ultimately leading to cost savings.

InsightMR Flow Unit

Design for on-line and in-line reaction monitoring, InsightMR flow unit is a PAT-ready solution that enables comprehensive analysis and confident decision-making directly under actual operating conditions and captures the start of the reaction.

InsightMR for High Field NMR

• Temperature controlled transfer lines: -20 to 120 °C capability
  
• Tested to withstand pressures over 10 bar
• Interchangeable glass tube. Proven robustness - industry tested
• Compatible with all Bruker sample changers
  
• 3- or 5-mm (Room Temperature and Cryo) Probes
• InsightMR 2.0 software.

InsightMR 2.0 is an advanced software platform for monitoring and analyzing chemical processes using on-line and in-line NMR. It combines integrated acquisition control with real-time data processing, allowing users to dynamically adjust acquisition parameters based on real-time kinetic data.

• Intuitive data acquisition with on-the-fly visualization of build-up curves
• Includes default experiments for both deuterated and non-deuterated solvents
• Efficient handling of large spectral datasets, including hundreds of stacked spectra
• Full support for 1D series (multiple nuclei), interleaved experiments, and parallel multi-sample analysis
• Automatic, seamless export to Mnova Reaction Monitoring Workstation, including phase correction, baseline correction, and GSD
• Results ready to report as kinetic plots and waterfall plots, with easy export options

• On-the-fly quantitative build-up curves without the need of tedious calibration steps 
• Structural information for reagents, (bi)products and intermediates 
• Increased selectivity, resolution and sensitivity compared to vibrational spectroscopy
• Used also to calibrate other techniques
• Straightforward installation and operation with minimal learning curve

Flow-NMR as a Process-Monitoring Tool for mRNA IVT Reaction

As mRNA vaccines expand beyond COVID-19, optimizing their production is critical. This work introduces flow-NMR as an advanced Process Analytical Technology (PAT) for real-time monitoring of in vitro transcription (IVT), the core step in mRNA synthesis. The approach enables faster, more efficient development of high-quality mRNA therapeutics.

https://doi.org/10.1016/j.xphs.2023.11.021

Ligand-Based Competition Binding by Real-Time 19F NMR in Human Cells

This study presents a novel real-time in-cell ¹⁹F flow-NMR approach to measure ligand binding affinities directly in living human cells. Using a fluorinated reference compound and flow-based acquisition, the method overcomes background interference and NMR invisibility of targets. Demonstrated with Hsp90α, it offers a powerful tool for early-stage drug development across a range of intracellular targets.

https://doi.org/10.1021/acs.jmedchem.3c01600

Monitoring Protein-Ligand Interactions in Human Cells by Real-Time Quantitative In-Cell NMR using a High Cell Density Bioreactor

This study showcases an NMR bioreactor that keeps human cells metabolically active for up to 72 hours, enabling real-time in-cell flow-NMR. The setup allows continuous monitoring of protein–ligand interactions and chemical modifications inside living cells. Combined with advanced data analysis, it provides kinetic insights crucial for early-stage drug development.

https://dx.doi.org/10.3791/62323

Determination of intracellular protein–ligand binding affinity by competition binding in-cell NMR

This study uses flow NMR technology to measure protein-ligand affinities within living cells. This real-time in-cell NMR bioreactor quantifies nanomolar dissociation constants (Kₑ) and maintains active cells for up to 72 hours. This innovation offers key insights into drug potency and specificity for early-stage drug development.

https://doi.org/10.1107/S2059798321009037

Live monitoring of cellular metabolism and mitochondrial respiration in 3D cell culture system using NMR spectroscopy

This study introduces a reproducible NMR bioreactor system for simultaneous metabolic and oxygenation monitoring in cells. By utilizing a simplified setup, it dynamically profiles metabolites and tracks mitochondrial respiration and oxygen consumption, providing enhanced insights into cellular metabolism.

https://doi.org/10.1039/D1AN00041A