Many free radicals species are highly reactive, with relatively short half-lives, and direct detection in solution by electron paramagnetic resonance (EPR) spectroscopy is challenging. Using spin traps and probes in EPR is an effective method that offers a solution to this challenge.
This webinar will provide an overview of two EPR techniques using spin traps and probes that enable detecting, identifying, and quantifying short-lived radicals such as reactive oxygen and nitrogen species (ROS and RNS). Spin traps are compounds that react covalently with highly transient free radicals to form relatively stable, persistent spin adducts that also possess paramagnetic resonance spectra detectable by EPR spectroscopy. In contrast to spin traps, spin probes do not ‘trap’ radicals, but are oxidized by ROS or RNS to form nitroxides (free radicals) with a half-life of several hours, which can readily be detected by EPR. Examples, sample preparation and data interpretation will be discussed.
Tuesday, May 17, 2022
10:00 AM in EDT / 4:00 PM CEST
Dr. Kalina Ranguelova
Senior EPR Applications Scientist, Bruker BioSpin
Dr. Ralph Weber
Senior EPR Applications Scientist, Bruker BioSpin