Discover the complex network of biological interactions that define biological processes from regulated homeostasis to disease pathology and progression within organs, tissues, and cellular systems.
Spatial localization of key protein expression targets is fundamental to the understanding and definition of disease, especially cancer biology, and single cell and bulk analysis for the characterization of cellular phenotypes.
Available tools for protein spatial profiling mostly come with limitations that can hinder building a complete picture of spatially defined biology such as intermediate plex levels that cannot track a sufficient number of proteins, limitide fields-of-view and inability to probe Omics layers outside of protein and RNA targets.
MALDI HiPLEX-IHC brings intact protein imaging to the next level by combining transcriptome relevant information from labelled protein expression mapping with advanced small molecule imaging from the same tissue section using MALDI Imaging. It is now possible to not only map proteins, but also examine metabolic activity and dig deeper into the biology governing cellular interactions, tissue homeostasis, and disease progression.
Multiplex. 100+ biomarkers, single scan, no cycling. Every probe comes with a unique mass tag.
Multimodal. Fluorescence microscopy, histopathological staining, and MALDI Imaging on one tissue section.
Multiomic. One workflow to visualize small molecules, lipids, glycans, intact proteins, and other macromolecules.
Fast and complete information. Survey an entire slide in 40 min. Drill down to ROIs at 5 µm high resolution with microGRID.
Easy Workflow. No conjugation. Routine IHC staining. No metal tags.
Widest Field-of-View in the Industry. No compromises on what part of the tissue section to image.
MALDI HiPLEX-IHC fits seamlessly into existing MALDI workflows for Bruker’s proven timsTOF fleX and rapifleX® platforms.
Full software automation through SCiLS™ autopilot allows for “pushbutton” workflow setup, ensuring reproducible and consistent preparation without the need for highly trained experts.
Immuno-oncology: Visualize the Tumor Microenvironment
Expand functionality into glycobiology
Neural Degeneration Profiling
Neuroscience: Mapping Molecular Structure for Translation
For Research Use Only. Not for use in clinical diagnostic procedures.