SLAS2023

February 25 - March 1, 2023

San Diego, CA, USA

SLAS2023

Join us at SLAS2023, where we showcase innovation that will redefine your analytical experience.

 

Meet us at booth #2219 to join our sessions.

Bruker Exhibitor Tutorial: Monday, February 27, 2023
12:00 - 1:00 pm (PST)

Location: Room 3 

 

In a nutshell: Bruker Arxspan cloud-based informatics solutions
Presented by: Adrienne Lemieux, Implementation and Service Specialist, Bruker Arxspan, USA

The Bruker Arxspan Suite is an integrated, highly scalable solution that supports both small molecule and biologics workflows, among others. Learn more from Adrienne how Bruker can support you with its latest informatics solution.

Adrienne Lemieux, Implementation and Service Specialist, Bruker Arxspan, USA

Six years of experience working in and managing a Neurobiology lab, with a focus on plasticity and mechanisms of the Drosophila melanogaster neuromuscular junction. Currently a Pre-Sales Implementation and Service Specialist at Bruker Arxspan.

Title: Bruker’s solution for Surface Plasmon Resonance (SPR)
Speaker: Sven Malik, Senior Application Scientist SPR, Bruker Daltonics SPR, Hamburg, Germany

Abstract:
Learn how SPR instruments empower scientists with many tools or ways to perform their work. The systems use a valve-less microfluidic system enabling fast transitions from sample to buffer, state-of-the-art sensitivity, and robustness to allow for the analysis of all types of samples, from ions to crude samples. High flexibility in assay development is ensured by individual sensor spot-addressing options.

Sven Malik, Senior Application Scientist SPR, Bruker Daltonics SPR, Hamburg, Germany

Sven studied Bioprocess Engineering at the Technical University of Dresden, Germany and graduated in 2012. During his diploma studies, he developed inverse assays for capture-molecules using surface plasmon resonance. Thereby, he focused on aptamers to establish a monitorable SELEX-process. Additionally, he investigated the interaction of cytomegalovirus protein pp65 against certain antibodies.

In 2012, he joined Sierra Sensors GmbH to start his role as application specialist. After 6 years he became senior application specialist as Bruker acquired Sierra Sensors GmbH. Currently he is involved in the customer support and product development.

Bruker Exhibitor Tutorial: Tuesday, February 28, 2023
12:00 - 1:00 pm (PST)

Location: Room 3 

 

In a nutshell: Bruker Arxspan cloud-based informatics solutions
Presented by: Adrienne Lemieux, Implementation and Service Specialist, Bruker Arxspan, USA

The Bruker Arxspan Suite is an integrated, highly scalable solution that supports both small molecule and biologics workflows, among others. Learn more from Adrienne how Bruker can support you with its latest informatics solution.

Adrienne Lemieux, Implementation and Service Specialist, Bruker Arxspan, USA

Six years of experience working in and managing a Neurobiology lab, with a focus on plasticity and mechanisms of the Drosophila melanogaster neuromuscular junction. Currently a Pre-Sales Implementation and Service Specialist at Bruker Arxspan.

Title: timsTOF MALDI PharmaPulse®: Reaching a New Level of Unbiased, Deep HTS by Label-Free Mass Spectrometry
Speaker: Arndt Asperger, Ph.D., Senior Applications Scientist, High Throughput Screening MALDI Mass Spectrometry, Bruker Daltonics, Bremen, Germany

Abstract:
In this tutorial, we provide insights to timsTOF MPP´s unique capabilities, i.e. accurate mass analysis at isotopic fidelity, ultrafast separation by trapped ion mobility spectrometry (TIMS), reproducible measurement of collisional cross-section (CCS) values, contribute to enhanced lead quality in (u)HTS in early drug discovery and accelerate HTE chemical synthesis monitoring in medchem/drug molecule design. Discover what’s new with MPP 2023 software and experience seamless timsTOF MPP integration in fully automated HTS environments that provides support for a variety of high-throughput application workflows such as mechanistic (u)HTS assays, ASMS for screening of protein-ligand interactions, compound library validation and HTE synthesis monitoring.

Arndt Asperger, Ph.D., Senior Applications Scientist, High Throughput Screening MALDI Mass Spectrometry, Bruker Daltonics, Bremen, Germany

Arndt Asperger, Ph.D., is working as a senior applications scientist at Bruker Daltonics GmbH & Co. KG, Bremen, Germany. Arndt is analytical chemist by training and got his Ph.D. from Leipzig University. He joined Bruker in 2002 and has been working in various positions related to MALDI-MS applications and applications development. His current focus is on applications development of MALDI based high-throughput MS methods.

Scientific Session: Tuesday, February 28, 2023
11:30 am - 12:00 pm (PST)

Location: Room 6C in  Session "Biochemical and Biophysical Assays"

 

Title: The Fast Track to Thermodynamics: Multiplexed Profiling with High Throughput Surface Plasmon Resonance
Speaker: Cyrill Brunner, Ph.D., Application Specialist, Bruker Switzerland, Fällanden, Switzerland

Abstract:
SPR is an established and widely used biophysical technology in screening and lead development campaigns for novel drug candidates. The real-time, label-free analysis of interactions offers additional insights into kinetics. Besides the value of a kinetic profile, the thermodynamic profile of potential lead candidate is equally important as it may give hints in steering the subsequent medicinal chemistry efforts to a more successful lead.

The thermodynamic profile of lead candidates is commonly determined with Isothermal Titration Calorimetry (ITC). This solution-based system is notoriously known for its low throughput of maximally a few dozen compounds per day and a high protein consumption, especially for weak binders such as lead candidates. Hence, ITC-measurements are often performed at a later stage in lead development with small numbers of molecules, thus, preventing insight into earlier candidates. 

We investigated the applicability of the multiplexing SPR Pro series for thermodynamic profiling of small molecules against proteins. Three different assay modes for a model case with a set of compounds against a single protein were analyzed first and the assay modes compared with respect to comparability and reproducibility. Subsequently, the thermodynamic profile of a set of eight compounds against three different proteins was determined in a single assay set-up. This information aided in understanding the underlying selectivity and affinity profile of the compound set in an automated approach with a single experiment. Consequently, the thermodynamic profile could be analyzed faster and at a substantially lower sample consumption of only 20-50 ug protein.

We herewith present an automated and high-throughput optimized approach for thermodynamic profiling of an interaction by SPR. Thus, the extension of typical parameters from a SPR experiment by a thermodynamic profile allows for a better understanding in lead development at an earlier stage than currently feasible.

Cyrill Brunner, Ph.D., Application Specialist, Bruker Switzerland, Fällanden, Switzerland

Dr. Cyrill Brunner is a trained pharmacist and obtained his Ph.D. at ETH Zurich in the group of Prof. Gisbert Schneider. His main background are numerous biophysical assays including SPR and computational drug design. He joined Bruker as application specialist in April 2020 and heads the SPR application lab in Fällanden, Switzerland.

Customer Sessions

Short Course: Sunday, February 26, 2023
10:30 am - 2:30 pm
Location: Room 17B

 

Title: Automated, Label-Free MALDI-MS-Based Cell assays: from Feasibility Study to High-Throughput Screening

Speakers:
Björn C. Fröhlich, Ph.D., Post-Doctoral Researcher, CeMOS, Baden-Wurttemberg, Germany
Steven Van Helden, Ph.D., Chief Technology Officer, Pivot Park Screening Centre, Noord-Brabant, Netherlands
Stefan Schmidt, Ph.D., Senior Research Manager, CeMOS, Baden-Wurttemberg, Germany


Scientific Session: Wednesday, March 1, 2023
1:00 pm - 1:30 pm
Location: Room 6C


Title: Evaluating target engagement of a focused lead-like and pan-assay interference compound collection using affinity selection mass spectrometry
Speaker: Saman Honarnejad, Ph.D., Chief Scientific Officer, Pivot Park Screening Centre, Netherlands

Electronic Laboratory Notebook – ArxSpan

The Bruker Arxspan Suite is an integrated, highly scalable solution that supports both small molecule and biologics workflows, among others.

The user-friendly, cloud-based module enables scientists to automatically capture, analyze, centralize and share device data and results. The centralizing of data creates a reference database of analytical results for synthesized compounds and impurities.

The module can be used with any lab instrument but is particularly suited to instruments that generate significant amounts of data. For example, NMR and Mass Spectrometers, SPR and QC data.

Don’t miss to listen to Adrienne Lemieux during our exhibitor tutorials on Monday and Tuesday 12:00 pm in room 3.

In a nutshell: Bruker Arxspan cloud-based informatics solutions
Presented by: Adrienne Lemieux, Implementation and Service Specialist, Bruker Arxspan, USA

Learn more from Adrienne how Bruker can support you with its latest informatics solutions.

Disclaimer

As you are certainly aware, special compliance regulations apply to public officials* and healthcare professionals** with regard to the event we are planning. If you accept our invitation, we will therefore assume that you will observe the compliance regulations that apply to you and that you have the necessary employer approval.

*Government Official means according the Bruker policies any of the following: any officer, employee or representative of a government (national, regional or local) entity, or any public agency, public authority, department or instrumentality thereof, regardless of their rank or title (e.g. a regulatory official or government inspector); any person working for or advising a government-owned or government-controlled enterprise (e.g. a professor at a government-owned university, or a purchaser at a government-owned hospital); any person working for or advising a national or international non-governmental organization (e.g. an employee of the Red Cross or The World Bank); any person performing a public function or providing a public service, even if that person works for a non-governmental institution (e.g. private security personnel working in public functions); any person hired to review or accept bids for a government agency; any person with the responsibility to allocate or expend government funds; any person in a public law function, civil servant, judge or military personnel; any person acting for a political party, including party officials, candidates or individuals holding a position in a political party office; members of royal families; or immediate family members of any of the persons listed above. An immediate family member is a grandparent, parent, spouse, significant other, child, or sibling.

**A Healthcare Professional (HCP) is in accordance with the Bruker policies any physician, dentist, nurse, pharmacist or other individual who may prescribe, administer, purchase, dispense, recommend, or supply medical products or treatments or pharmaceutical products. In many cases, Bruker interacts with HCPs who work for state-owned hospitals (e.g. as medical scientists). These individuals will be classified as both HCPs and Government Officials.

 

For Research Use Only. Not for use in clinical diagnostic procedures.