FEATURING EXCLUSIVE PRESENTATIONS ON:
High-speed atomic force microscopy (HS-AFM) is a powerful technique that provides dynamic movies of biomolecules at work .
To break current temporal limitations to characterize molecular dynamics using HS-AFM, we developed HS-AFM height spectroscopy (HS-AFM-HS), a technique whereby we oscillate the HS-AFM tip at a fixed position and detect the motions of the molecules under the tip. This gives sub-nanometer spatial resolution combined with microseconds temporal resolution of molecular fluctuations. HS-AFM-HS can be used in conjunction with HSAFM imaging modes, thus giving access to a wide dynamic range ...
Recent atomic force microscopy (AFM) technology developments have led to unprecedented imaging rates in fluid, setting new milestones in high-speed scanning capabilities. Bruker recently launched the fastest commercially available high-speed AFM (NanoRacer®) able to reach a scanning speed of 50 frames/sec at 5000 lines/sec. High-speed AFM not only delivers atomic resolution but also enables the true, real-time visualization of time-resolved dynamics associated with cellular processes and the binding mechanisms of individual biomolecules. For example, the dynamics of individual protein binding behavior, two-dimensional protein assemblies, motor proteins, membrane trafficking, structural transitions of nucleic acids, can now be observed.
The WHO reports about 3.4M cases of breast and prostate cancer each year, resulting in about a million deaths worldwide. The majority of the deaths are attributed to metastasis, the process of translocation, and recolonization of cancer to a remote site. Both prostate cancer and breast cancer exhibit a strong propensity to metastasize to bone, at which point the prognosis for patients is poor. Cancer, when diagnosed at the primary site, is treatable. However, more than 90% of cancer-related deaths result from metastasis. We have developed a novel bone mimetic nanoclay based scaffold to generate bone using the tissue engineering approach [1-5]. Seeding of commercial prostate and breast cancer cells on the tissue engineered bone is used to generate tumors [6, 7]. The tumors are investigated for mechanical properties using the direct nanoindentation technique...
In this study we investigated structural changes in collagen fibrils in human AAA tissue extracted at the time of vascular surgery and in aorta extracted from angiotensin II (AngII) infused ApoE−/− mouse model of AAA. Collagen fibril structure was examined using transmission electron microscopy and atomic force microscopy (AFM). Images were analyzed to characterize the length and depth of D-periodicity, fibril diameter and fibril curvature. Tissues were also stained using a novel reagent, collagen hybridizing peptide (CHP) (which stains degraded collagen) and analyzed using a combination of AFM and fluorescent microscopy. Our results elucidate how abnormal collagen fibrils with compromised D-periodic banding were observed in the excised human tissue and in remodeled regions of AAA in AngII infused mice...
The oral cavity supports the growth of a diverse range of bacteria with its relatively stable environmental conditions and stream of nutrients derived from salivary components, gingival crevicular fluid and host diet. Oral biofilm formation on dental surfaces initiates with the attachment of primary colonizers followed by the attachment of secondary and later colonizers. Understanding how primary colonizers invade oral surfaces can be challenging but remain necessary for the development of new anti-microbial/anti-biofilm strategies. In this presentation, Dr. Bozec will review some of his team’s recent work on the biophysical characterization of bacterial species as well as the early-adhesions of oral bacteria/fungi on relevant surfaces characterized by single-cell force spectroscopy. In a second part of this presentation, Dr. Bozec will demonstrate how the combined used of Atomic Force Microscopy and Optical Coherence Tomography can be used pertinently to investigate the structure-property relationships in oral biofilms.
Detecting mechanical properties of the intact skin in-vivo leads to a novel quantitative method to diagnose skin diseases and to monitor skin conditions in clinical settings. Current research and clinical methods that detect skin mechanics have major limitations. The in-vitro experiments are done in non-physiological conditions and in-vivo clinical methods measure unwanted mechanics of underneath fat and muscle tissues but report the measurement as skin mechanics. An ideal skin mechanics should be captured at skin scale (i.e., micron-scale) and in-vivo. However, extreme challenges of capturing the in-vivo skin mechanics in micron-scale including skin motion due to heart beep, breathing and movement of the subject, has hindered measurement of skin mechanics in-vivo...
Simon Scheuring, Ph.D., Professor of Physiology and Biophysics in Anesthesiology, Weill Cornell Medicine
Kalpana Katti, Ph.D., University Distinguished Professor, North Dakota State University
Gunjan Agarwal, Ph.D., Professor, Mechanical and Aerospace Engineering, Ohio State University
Laurent Bozec, Ph.D., Professor (Associate), Faculty of Dentistry, University of Toronto
Mojtaba Azadi, Ph.D., Associate Professor, San Francisco State University
Andreas Kraus, Ph.D., Application Scientist, Bruker
Heiko Haschke, Ph.D., Application Scientist, Bruker