During the live event, Dr. Ichikawa will present his latest work using advanced techniques, including 3D ex-vivo culture, light-sheet microscopy, and photomanipulation, to study peri-implantation development in mice.
Find out more about the technology featured in this webinar or our other solutions for light-sheet microscopy:
Upon implantation, mammalian embryos undergo major morphogenesis and key developmental processes, such as body axis specification. To gain mechanistic insights into their development, we created a 3D ex-vivo culture, which not only recapitulates mouse peri-implantation development from E4.5 to E6.0, but also enables us to monitor in-toto by using an inverted light-sheet microscope, specifically Bruker's InVi SPIM. Our imaging modality accommodates multi-samples for 48 hours of imaging without compromising the development of the embryos, and offers sufficient spatiotemporal resolution for quantitative image analysis, including membrane segmentation. Furthermore, the inverted InVi SPIM has perturbation tools, such as photomanipulation, that allows us to reveal a key cellular mechanism during peri-implantation development. This ex-vivo investigation ultimately will lead to a finer mechanistic understanding of the crucial period of mammalian development.
Takafumi Ichikawa, Ph.D. Assistant Professor, Kyoto University