Light-Sheet Images in Developmental Biology
Embryogenesis & Developmental Biology
Drosophila Embryo Development
Transgenic line expressing His2Av-mCherry as fluorescent nuclear reporter. The fruit fly embryo was imaged for almost one complete day (4 × 200 slices every 30 seconds). Imaged on the MuVi SPIM.
Courtesy of:
Lars Hufnagel
European Molecular Biology Laboratory (EMBL)
Heidelberg, Germany
Cell Tracking in Drosophila Embryo Development
Cell tracking created with arivis Vision4D 3D visualization and analysis software. Imaged on the MuVi SPIM.
Courtesy of:
Celia Smits and Stanislav Y. Shvartsman
Department of Molecular Biology
Princeton University, NJ
USA
Zebrafish Development
Zebrafish imaged on the MuVi SPIM. Stitched from 5 stacks in Imaris, each 340 slices, 16 hour/10min. Fish growth can be observed.
Courtesy of:
Prof. Jingxia Liu
Huazhong Agricultural University
China
Zebrafish Embryonic Development
Zebrafish embryo expressing Histone H2A-GFP imaged every 6 min from late gastrula to 15–17 somite stage. Imaged on the MuVi SPIM.
Courtesy of:
Andres Collazo
Caltech, Pasadena, CA, USA
as well as: Course faculty and participants of the 2017 Zebrafish Course
Marine Biological Laboratory (MBL)
Woods Hole, MA, USA
Vascular Development in Zebrafish
From left to right: the video shows a beating Zebrafish heart imaged at 50 frames/sec, followed by Zebrafish blood vessels (magenta) and red blood cells (yellow) and Zebrafish blood flow imaged at 50 frames/sec. Imaged on the MuVi SPIM.
Courtesy of:
Nadia Mercader & Inés Marques
University of Bern
Bern, Switzerland
Drosophila Egg Chambers
Drosophila ovariole stained with phalloidin to label actin (red) found along membranes and the germline ring canals, DAPI (blue) to show the nuclei, and a somatic ring canal marker (green) to label the ring canals in the epithelium.
Courtesy of:
Jasmin Imran Alsous
Schvartsman Lab
Princeton University, Princeton, NJ, USA
