Light-Sheet Fluorescence Microscopy for Living Plants

_{KEYWORDS: Plant Biology; Case Study; Light-Sheet Fluorescence Microscopy; Image Acquisition and Data Analysis}

Light-sheet fluorescent microscopy (LSFM) is well-suited to overcome many of the complications around imaging live and delicate plants. Because of this capability, researchers use LSFM to create compelling data about plants through innovative image acquisition and data processing.  

This application note, “Light-Sheet Fluorescence Microscopy for Living Plants,” discusses the importance of using light-sheet fluorescence microscopy to overcome many of the challenges associated with imaging plants. Learn about the technical components of a light-sheet system – including light-sheet thickness and aperture – and data processing solutions for creating compelling data. Real-world examples and figures also explain how to use these components to study plant dynamics (growth) and structures (cell walls and tissues).   

Readers can expect to learn more about:

• What are the challenges of plant imaging;
• How to overcome these challenges using LSFM;
• Challenges to processing compelling data;
• Case study of using LSFM for investigating ANNEXIN 1 in Arabidopsis.