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Fluorescence Microscopy Library

Webinar Recap: Nano-organization of Spontaneous GABAergic Transmission Directs its Autonomous Function in Neuronal Signaling

This webinar recap summarizes a presentation given by Natalie Guzikowski, a student and member of Kavalali Lab at Vanderbilt University about the lab’s work combining super-resolution microscopy with other recording and imaging methods to investigate fundamental questions about how, where, and why synaptic transmission occurs.

The Kavalali lab has an interest in exploring protein influence on neurotransmitter release and postsynaptic signaling at inhibitory synapses. In this webinar, Guzikowski provides a detailed explanation of the lab's use of a novel multi-modal approach to probe the structural and functional nano-organization of the GABAergic synapse. 

The presentation includes discussion of the lab's methods and findings, including the various techniques their lab used to create 3D reconstructions of pre- and post synaptic clusters and identify gephyrin molecule organization, as well as how these methods reveal new information about inhibition's role in neural circuits. The presentation is followed by a Q&A session with members of the live audience.

Download the webinar recap or watch the recording to learn more about these and other topics.

Download Recap (PDF, 299 KB)
FROM THE WEBINAR

Audience Q&A

Download the webinar recap or watch the recording to learn more about these and other topics.

  1. How is super-resolution microscopy used to probe the nano-organization of Spontaneous GABAergic Transmission?
  2. What benefits did using dSTORM provide?
  3. What types of reconstructions were made using localization data?
  4. What is the purpose of focusing on the specific interaction of gephyrin and antimalarial artemisinins?
  5. What changes were observed in gephyrin clusters after treatment with artemisinin?
  6. How did the treatment with artemisinin affect neurotransmission?
  7. How was neurotransmission organized at the GABAergic post-synapse?

If you missed this event, you can watch the recording here: