DNA-PAINT is an advanced single-molecule localization microscopy (SMLM) technique that supports multiplexed imaging of fluorescently tagged molecules at the highest spatial resolution currently achievable.
Like other SMLM techniques, DNA-PAINT makes it possible to find the position of each molecule with high precision. Yet the nature of DNA-PAINT and its process of execution uniquely enable sequential labeling of an unlimited number of structures of interest within a sample (with essentially no photobleaching) and make it easier and more efficient to implement than other techniques.
This article focuses on the principles, capabilities, and unique advantages of DNA-PAINT. Readers can expect to learn:
KEYWORDS: advantages of DNA-PAINT, single-molecule localization microscopy, SMLM techniques, multiplexed imaging, molecular imaging