Exploring DNA-PAINT

DNA-PAINT is an advanced single-molecule localization microscopy (SMLM) technique that supports multiplexed imaging of fluorescently tagged molecules at the highest spatial resolution currently achievable.

Like other SMLM techniques, DNA-PAINT makes it possible to find the position of each molecule with high precision. Yet the nature of DNA-PAINT and its process of execution uniquely enable sequential labeling of an unlimited number of structures of interest within a sample (with essentially no photobleaching) and make it easier and more efficient to implement than other techniques.

This article focuses on the principles, capabilities, and unique advantages of DNA-PAINT. Readers can expect to learn:

• What super-resolution imaging with DNA-PAINT is;
• How it differs from and compares to other SMLM imaging techniques;
• The principles and steps of super-resolution imaging with DNA-PAINT; and
• The value of DNA-PAINT for current and future life science research.

_{KEYWORDS: advantages of DNA-PAINT, single-molecule localization microscopy, SMLM techniques, multiplexed imaging, molecular imaging}