Super-Resolution Microscopes

Vutara VXL

More than the leading single-molecule localization microscope (SMLM). A benchtop multimodal bioimaging workstation designed for your lab.
Vutara VXL

Highlights

Vutara VXL

The Vutara VXL comprehensive biological workstation for nanoscale biological imaging opens an affordable and easy-to-use path for both core facilities and individual investigators to enter the world of super-resolution imaging by incorporating Bruker’s industry-leading single-molecule localization microscopy (SMLM) technology in a streamlined system with compact footprint. The new system enables research on DNA, RNA and proteins, from macromolecular complexes and super-structures, to chromatin structure and chromosomal substructures, to studying functional relationships in genomes and in various subcellular organelles. This novel system also supports advanced spatial biology research in extracellular matrix structures, extracellular vesicles (EV), virology, neuroscience, and live-cell imaging. When combined with Bruker’s unique microscope fluidics unit, Vutara VXL enables multiplexed imaging for targeted, sub-micrometer multiomics in genomics, transcriptomics, and proteomics research.

Deepest
3D imaging
Achieves depths of >30 μm imaging with proprietary biplane technology.
Seamless
multimodal imaging
Combines with robust, flexible software for studies across length scales.
Integrated
microfluidics
Provides easy yet unlimited multiplexed imaging for spatial genomics, transcriptomics, and proteomics.

Features

Features

Every Acquisition Is 3D

Proprietary biplane technology, combined with a spatial filter in the emission light path, allows you to acquire 3D data with every acquisition. For thicker specimens, the Vutara allows you to easily perform a Z series and automatically localizes and reconstructs the entire volume.

Single-Molecule Imaging Beyond the Cover Slip

The Vutara VXL is capable of imaging far from the surface of the coverslip to accommodate a wide range of sample types. Thanks to the proprietary biplane technology, the Vutara VXL with the SRX software can perform single-molecule localization microscopy on more sample types than any other commercial single-molecule localization microscope available. Making cultured cells, cell colonies, tissue sections, and entire model organisms accessible for your single-molecule localization experiments.

Single-molecule sample types.

Turn Localizations Into Information

Vutara’s Quantitative Localization Microscopy suite allows you to turn localizations into meaningful results. Vutara's SRX workflow-driven software guides users through the setup, calibration, imaging, processing, and analysis of the super-resolution single-molecule localization experiment. The SRX software combines real-time localization processing with powerful 3D visualization and analysis tools to let researchers quickly create publication quality videos, images, and measurements.

Specifications

Specifications

Imaging Modalities
Super-resolution localization microscopy (SMLM)
  • SMLM with high z-resolution for STORM, PALM, PAINT, and related super-resolution applications
  • Localization microscopy with large FOV, optimized for super-resolution multiplexed genomics applications
Widefield microscopy
  • Epi-fluorescence microscopy with large FOV
  • Transmitted light microscopy with large FOV
Imaging Optics
Excitation lasers (nominal laser power at diode)
  • 405 nm, 120 mW
  • 488 nm, 2000 mW (optional)
  • 555 nm, 2000 mW
  • 638 nm, 1200 mW
  • 750 nm, 1500 mW (optional)
Flat illumination
  • Flat excitation profile guaranteed by top hat illumination from a square fiber
Multi-color acquisition
  • Up to 5 colors sequential
Multi-plane imaging
  • Simultaneous imaging of two focal planes allows 1 µm depth discrimination (larger z range possible in z-stack mode)

Camera

  • Orca Flash 4.0 V3 sCMOS camera
  • Orca Fusion BT sCMOS camera (optional)
Objective
  • 60x magnification
  • 1.3 numerical aperture (NA)
  • Silicon oil immersion
  • 0.3 mm working distance
  • #1.5H cover glass (0.170 ±0.005 mm)
Field of View (FOV)
  • 200 µm x 200 µm for multiplexed localization microscopy and widefield imaging
  • 50 µm x 50 µm for SMLM with switching (STORM, PALM, PAINT) and 3D localization (biplane detection)
  • Larger FOV with tile scanning
Single Molecule Localization (SMLM)
3D SMLM method
  • Fitting of measured PSF to maximize accuracy in xyz
  • Proprietary biplane for high accuracy z-resolution and deep sample imaging
SMLM resolution
  • 20 nm laterally (xy)
  • 50 nm axially (z) with biplane
Imaging depth
  • > 30 µm (typical, dependent on sample)
Stage, Focus Drive, and Sample Holder
xy-stage
  • SMLM with high z-resolution for STORM, PALM, PAINT, and related super-resolution applications
  • Localization microscopy with large FOV, optimized for super-resolution multiplexed genomics applications
z-focus
  • Course focus to localize sample
  • Fine focus for fast z-stack acquisition
Drift correction
  • Active focus drift correction during data acquisition
  • Focus drift <30 nm over 10 minutes
  • xy drift correction post-acquisition
Environmental control
  • Live cell incubation with humidity, CO2, and temperature control (optional)
Multiplexing (optional)
Microfluidics unit for sequential labeling in localization microscopy applications
  • Multiplexed exchange of sample buffer (optional fluidics unit)
  • 15 x 2 ml reservoirs for imaging probes
  • 2 x 15 ml, 1 x 50 ml, 1 x 100 ml for washing and buffer solutions
  • 1 µL/min – 1000 µL/min flow rate
  • Seamless integrations in SRX microscope control software
Dimensions and Environment
Compact table-top design
  • Space required if microscope and laser launch are installed on same table: 120 cm x 75 cm (4 ft x 2.5 ft)
  • Microscope without camera:

                  34 cm x 40 cm x 34 cm, (1.2 ft x 1.3 ft x 1.2 ft)

                  35 kg (78 lbs)

  • Laser launch:

                  33 cm x 53 cm x 32 cm (1.0 ft x 1.7 ft x 1.0 ft)

                  30 kg (65 lbs)

  • Electronics box stacked on top of laser launch:

                  53 cm x 35 cm x 14 cm (1.7 ft x 1.1 ft x 0.4 ft)

                  8 kg (17 lbs)

Operating in a typical laboratory environment
  • The light-tight design does not require dimmed room light
Vibration insulation included
  • The system includes vibration insulation (sturdy table or bench required for installation)
  • Optional: Customers can supply optical table for challenging environments
Software
Workflow oriented software for easy data acquisition
  • SMLM modalities

                  STORM / dSTORM

                  PALM

                  PAINT

                  Other blinking-based modalities

  • Genomics modalities

                  Chromatin tracing

                  smFISH (in development)

  • Multiplexing workflow

                  Tracking of probe cycles and positions

                  Support for an unlimited number of probes

  • Calibration workflow to ensure superior localization precision
SMLM processing
  • xy fitting based on measured point-spread function (PSF)
  • z position determined from calibrated biplane data
  • Initial localization during data acquisition
  • Statistical tools to validate localizations
Particle tracking
  • Diffusion analysis based on nearest neighbors
  • Particle assignment based on sparse emitter tracking
  • Mean-square displacement and distribution analysis and plots
  • Mean velocity and angle calculations and plots
Fluidics Manager
  • Setup and control multiplexed imaging workflow
  • Tracking of probe labels and metadata
Chromatin tracing (optional)
  • Optical reconstruction of chromatin architecture

Drift correction

  • Auto-correlation
  • Fiducials embedded in the sample
Statistical analysis tools
  • Spatial distribution

                  Ripley’s K functions

                  Pair correlation

                  Nearest neighbors

  • Cluster analysis

                  DBSCAN

                  OPTICS

                  Delaunay

                  Particle counts

                  Volume and density calculations

                  Determination of radius of gyration and sphericity

                  Principal component analysis (PCA)

                  Calculation of centroid

  • Colocalization

                  Pearson’s correlation coefficient

                  Mander’s overlap coefficient

                  Intensity correlation quotient

                  Cross pair correlation

                  Joint histogram

                  STORM-RLA

  • Resolution analysis

                  Fourier ring correlation

                  Labeling density resolution analysis

                  Local resolution correlation mapping

                  Nearest neighbors’ analysis

Integrated visualization
  • 2D and 3D visualizations
  • Point cloud and point splatting
  • Volumetric slices
  • Isosurface
  • Maximum intensity rendering
  • Depth color coding
  • Time frame color coding
  • Color coding by probe
  • Cluster hull visualization
  • Wireframe visualization of clusters
  • Trajectories for tracked particles
Open data formats
  • Raw images are stored in highly efficient binary format and can be exported in ome-tiff format
  • Export of drift corrected data
  • Localizations are stored in highly efficient binary format and can be exported as .csv or MATLAB binary files
  • Results from cluster analysis are stored and can be exported
  • Export of results from statistical analysis as .csv or MATLAB binary files
  • All imaging and processing settings are stored in xml or Jason format
  • Images can be exported as vector graphics or user-defined size/DPI
  • Specifications for all data formats are published
Additional Data Storage
Network attached storage (NAS) unit (optional)
  • 90 TB effective storage
  • 2 Gb/sec transfer rate

Software

A Complete Solution, From Acquisition to Analysis

With SRX software and its Quantitative Localization Microscopy analysis suite, Vutara VXL can provide visual and quantitative information from biological samples. By localizing individual molecules, Vutara can generate stunning 3D images while simultaneously providing in-depth quantitative analysis tools.

SRX software.

Webinars

Testimonials

With more than 15 years of experience in designing microscopes in academia and in industry, I am proud to contribute to the development of Vutara's super-resolution microscopes. With biplane 3D detection and fast sCMOS imaging, Vutara has the most advanced super-resolution microscope on the market.

Joerg Bewersdorf, Ph.D., Yale University

After scanning the market for super-resolution microscopes and personally visiting and testing most commercially available systems with our own samples, I can say that I am most impressed with Vutara's SR-350. In particular, the user friendliness of their imaging software as well as the 3D capability in super-resolution mode impressed me.

Vutara has an excellent support team and staff as a whole making our transition to super-resolution a well-supported experience. If you're looking to advance your research by super resolution microscopy, I can confidently say that Vutara's systems are an excellent choice.

Thomas Stroh, Ph.D., Core Facility Director, McGill University

The volume of data you can acquire on a super-resolution system is incredible when comparing electron microscopy especially considering the sample preparation.

Peter McPherson, Ph.D., McGill University

Having published with a Vutara super-resolution system, I can confidently say that they offer one of the most advanced super-resolution microscopes available. Their attention to detail and ongoing close collaboration makes them one of my preferred microscopy vendors.

Brigitte Ritter, Ph.D., Boston University

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