Super-Resolution Microscopes

Vutara VXL

Revolutionize your lab with the ultimate multimodal bioimaging super-resolution microscope.

Vutara VXL

The Bruker Vutara VXL super-resolution microscope enables researchers to enter the world of super-resolution imaging with ease. It incorporates Bruker’s industry-leading single-molecule localization microscopy (SMLM) technology in a streamlined system with a compact footprint. The Vutara VXL system allows you to perform research on DNA, RNA, and proteins, from macromolecular complexes and super-structures to chromatin structure and chromosomal substructures, as well as studying functional relationships in genomes and various subcellular organelles. This novel system also supports advanced spatial biology research in extracellular matrix structures, extracellular vesicles (EV), virology, neuroscience, and live-cell imaging. When combined with Bruker's unique microscope fluidics unit, Vutara VXL enables multiplexed imaging for targeted, sub-micrometer multiomics in genomics, transcriptomics, and proteomics research.

3D imaging
Achieves depths of >50 μm imaging with proprietary biplane technology.
multimodal imaging
Utilizes flexible software to quantitatively analyze specimens with greater depth.
Provides easy yet unlimited multiplexed imaging for spatial genomics, transcriptomics, and proteomics.

Every Acquisition Is 3D

The Vutara VXL is equipped with proprietary bi-plane technology that enables you to gather 3D data with every acquisition. This technology allows you to easily perform a Z-stack series for thicker specimens and automatically localize and reconstruct the entire volume. With Vutara VXL, you can obtain comprehensive 3D data of your samples with ease.

Single-Molecule Imaging Beyond the Cover Slip

The Vutara VXL is capable of imaging far from the surface of the coverslip, about 50 µm deep, to accommodate a wide range of sample types. Thanks to the proprietary biplane technology, the Vutara VXL with the SRX software can perform single-molecule localization microscopy on more sample types than any other commercial single-molecule localization microscope available. Making cultured cells, cell colonies, tissue sections, and entire model organisms accessible for your single-molecule localization experiments.

Single-molecule sample types, ~50 µm deep.

Turn Localizations Into Information

Vutara’s Quantitative Localization Microscopy suite enhances your productivity and allows you to turn localizations into meaningful results. Vutara's SRX workflow-driven software guides users through the setup, calibration, imaging, processing, and analysis of the super-resolution single-molecule localization experiment. SRX boosts the value of the system by providing dedicated software that is designed specifically for SMLM. The SRX software combines real-time localization processing with advanced visualization and sophisticated quantitative analysis tools to let researchers quickly create publication quality videos, images, and measurements.


Imaging Modalities
Super-resolution localization microscopy (SMLM)
  • SMLM with high z-resolution for STORM, PALM, PAINT, and related super-resolution applications
  • Localization microscopy with large FOV, optimized for super-resolution multiplexed genomics applications
Widefield microscopy
  • Epi-fluorescence microscopy with large FOV
  • Transmitted light microscopy with large FOV
Imaging Optics
Excitation lasers (nominal laser power at diode)
  • 405 nm, 120 mW
  • 488 nm, 2000 mW (optional)
  • 555 nm, 2000 mW
  • 638 nm, 1200 mW
  • 750 nm, 1500 mW (optional)
Flat illumination
  • Flat excitation profile guaranteed by top hat illumination from a square fiber
Multi-color acquisition
  • Up to 5 colors sequential
  • 2 colors simultaneously with 2 cameras (optional)
Multi-plane imaging
  • Simultaneous imaging of two focal planes allows 1 µm depth discrimination (larger z range possible in z-stack mode)
  • Orca Flash 4.0 V3 sCMOS camera
  • Orca Fusion BT sCMOS camera (optional)
  • 60x magnification
  • 1.3 numerical aperture (NA)
  • Silicon oil immersion
  • 0.3 mm working distance
  • #1.5H cover glass (0.170 ±0.005 mm)
Field of View (FOV)
  • 200 µm x 200 µm for multiplexed localization microscopy and widefield imaging
  • 50 µm x 50 µm for SMLM with switching (STORM, PALM, PAINT) and 3D localization (biplane detection)
  • Larger FOV with tile scanning
Single Molecule Localization (SMLM)
3D SMLM method
  • Fitting of measured PSF to maximize accuracy in xyz
  • Proprietary biplane for high accuracy z-resolution and deep sample imaging
SMLM resolution
  • Better than 20 nm laterally (xy)
  • 50 nm axially (z) with biplane
Imaging depth
  • > 50 µm (typical, dependent on sample)
Stage, Focus Drive, and Sample Holder
  • SMLM with high z-resolution for STORM, PALM, PAINT, and related super-resolution applications
  • Localization microscopy with large FOV, optimized for super-resolution multiplexed genomics applications
  • Course focus to localize sample
  • Fine focus for fast z-stack acquisition
Drift correction
  • Active focus drift correction during data acquisition
  • Focus drift <30 nm over 10 minutes
  • xy drift correction post-acquisition
Environmental control
  • Live cell incubation with humidity, CO2, and temperature control (optional)
Multiplexing (optional)
Microfluidics unit for sequential labeling
  • Multiplexed exchange of sample buffer (optional fluidics unit)
  • 15 samples + buffer x 2 ml reservoirs for imaging probes
  • 96 samples + buffer
  • Seamless integrations in SRX microscope control software
Dimensions and Environment
Compact table-top design
  • Space required if microscope and laser launch are installed on same table: 120 cm x 75 cm (4 ft x 2.5 ft)
  • Microscope without camera:

                  34 cm x 40 cm x 34 cm, (1.2 ft x 1.3 ft x 1.2 ft)

                  35 kg (78 lbs)

  • Laser launch:

                  33 cm x 53 cm x 32 cm (1.0 ft x 1.7 ft x 1.0 ft)

                  30 kg (65 lbs)

  • Electronics box stacked on top of laser launch:

                  53 cm x 35 cm x 14 cm (1.7 ft x 1.1 ft x 0.4 ft)

                  8 kg (17 lbs)

Operating in a typical laboratory environment
  • The light-tight design does not require dimmed room light
Vibration insulation included
  • The system includes vibration insulation (sturdy table or bench required for installation)
  • Optional: Customers can supply optical table for challenging environments
Workflow-defined software for easy data acquisition
  • SMLM modalities

                  STORM / dSTORM



                  Other blinking-based modalities

  • Genomics modalities

                  Chromatin tracing


  • Multiplexing workflow

                  Tracking of probe cycles and positions

                  Support for an unlimited number of probes

  • Calibration workflow to ensure superior localization precision
SMLM processing
  • xy fitting based on measured point-spread function (PSF)
  • z position determined from calibrated biplane data
  • Initial localization during data acquisition
  • Statistical tools to validate localizations
Particle tracking
  • Diffusion analysis based on nearest neighbors
  • Particle assignment based on sparse emitter tracking
  • Mean-square displacement and distribution analysis and plots
  • Mean velocity and angle calculations and plots
Fluidics Manager
  • Setup and control multiplexed imaging workflow
  • Tracking of probe labels and metadata

Drift correction

  • Auto-correlation
  • Fiducials embedded in the sample
Statistical analysis tools
  • Spatial distribution

                  Ripley’s K functions

                  Pair correlation

                  Nearest neighbors

  • Cluster analysis




                  Particle counts

                  Volume and density calculations

                  Determination of radius of gyration and sphericity

                  Principal component analysis (PCA)

                  Calculation of centroid

  • Colocalization

                  Pearson’s correlation coefficient

                  Mander’s overlap coefficient

                  Intensity correlation quotient

                  Cross pair correlation

                  Joint histogram


  • Resolution analysis

                  Fourier ring correlation

                  Labeling density resolution analysis

                  Local resolution correlation mapping

                  Nearest neighbors’ analysis

Chromatin tracing (optional)
  • Optical reconstruction of chromatin architecture
Integrated visualization
  • 2D and 3D visualizations
  • Point cloud and point splatting
  • Volumetric slices
  • Isosurface
  • Maximum intensity rendering
  • Depth color coding
  • Time frame color coding
  • Color coding by probe
  • Cluster hull visualization
  • Wireframe visualization of clusters
  • Trajectories for tracked particles
Open data formats
  • Raw images are stored in highly efficient binary format and can be exported in ome-tiff format
  • Export of drift corrected data
  • Localizations are stored in highly efficient binary format and can be exported as .csv or MATLAB binary files
  • Results from cluster analysis are stored and can be exported
  • Export of results from statistical analysis as .csv or MATLAB binary files
  • All imaging and processing settings are stored in XML or JSON format
  • Images can be exported as vector graphics or user-defined size/DPI
  • Specifications for all data formats are published
Additional Data Storage
Network attached storage (NAS) unit (optional)
  • 90 TB effective storage
  • 2 Gb/sec transfer rate

A Complete Solution, From Acquisition to Analysis

With SRX software and its Quantitative Localization Microscopy analysis suite, Vutara VXL can provide visual and quantitative information from biological samples. By localizing individual molecules, Vutara can generate stunning 3D images while simultaneously providing in-depth quantitative analysis tools.

SRX software.

With more than 15 years of experience in designing microscopes in academia and in industry, I am proud to contribute to the development of Vutara's super-resolution microscopes. With biplane 3D detection and fast sCMOS imaging, Vutara has the most advanced super-resolution microscope on the market.

Joerg Bewersdorf, Ph.D., Yale University

After scanning the market for super-resolution microscopes and personally visiting and testing most commercially available systems with our own samples, I can say that I am most impressed with Vutara's SR-350. In particular, the user friendliness of their imaging software as well as the 3D capability in super-resolution mode impressed me.

Vutara has an excellent support team and staff as a whole making our transition to super-resolution a well-supported experience. If you're looking to advance your research by super resolution microscopy, I can confidently say that Vutara's systems are an excellent choice.

Thomas Stroh, Ph.D., Core Facility Director, McGill University

The volume of data you can acquire on a super-resolution system is incredible when comparing electron microscopy especially considering the sample preparation.

Peter McPherson, Ph.D., McGill University

Having published with a Vutara super-resolution system, I can confidently say that they offer one of the most advanced super-resolution microscopes available. Their attention to detail and ongoing close collaboration makes them one of my preferred microscopy vendors.

Brigitte Ritter, Ph.D., Boston University

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