PASEF® (Parallel Accumulation Serial Fragmentation) is an innovative mass spectrometry technology that revolutionizes proteomics analysis. PASEF® is uniquely incorporated into Bruker's timsTOF mass spectrometers, which combine orthogonal ion mobility and time-of-flight separation to achieve unprecedented levels of selectivity, speed, and sensitivity.
PASEF® is a versatile technology that can be used for a wide range of applications, including:
Proteome-wide analysis: PASEF® can be used to identify and quantify low-abundance peptides in a sample.This can be used to gain a comprehensive understanding of the proteome and identify potential biomarkers for disease.
Post-translational modification (PTM) analysis: PASEF® can be used to identify and quantify PTMs, which are chemical modifications that can alter the function of proteins. This can be used to study the role of PTMs in disease and to develop new therapeutic targets.
Small molecule analysis: PASEF® can be used to identify and quantify small molecules, such as metabolites and lipids. This can be used to study the metabolism of cells and tissues and to identify potential biomarkers for disease.
Immunopeptidomics: PASEF® scan modes provide immuno-researchers with the ability to identify the largest depth of these low abundant peptides at the highest throughput. Uniquely, PASEF® scan modes allow for the selective inclusion of the regions of the precursor ion cloud rather than indiscriminate exclusion based on charge state. This allows access to both single-charge and higher-charge state peptide precursors in a single analysis while ignoring single-charge contaminant ions, leading to unprecedented depths of coverage in immunopeptidomics.
Single-cell analysis: PASEF® can be used to analyze the proteome of individual cells. This can be used to study the heterogeneity of cells within a tissue and to identify potential biomarkers for cancer.
Lipidomics: PASEF® can be used to identify and quantify lipids, which are essential components of cell membranes and signaling molecules. This can be used to study the role of lipids in health and disease.
Cross-linking: PASEF® can be used to study the structure of proteins that have been cross-linked, which can be used to understand protein-protein interactions and protein folding.
Glycomics and Glycoconjugates: PASEF® can be used to identify and quantify glycoforms, which are sugar molecules that are covalently attached to proteins. This can be used to study the role of glycans in health and disease.
PASEF® (Parallel Accumulation Serial Fragmentation) is a revolutionary mass spectrometry technique rewriting the rules of proteome analysis. It leverages the unmatched power of time and space focusing, combining orthogonal ion mobility (IM) and time-of-flight (TOF) separations to deliver unprecedented selectivity, speed, and sensitivity.
Unlike traditional methods, PASEF® exploits the unique Collisional Cross Section (CCS) property of peptides, a measurable fingerprint differentiating them based on size and shape. This "space focusing" via IM separates peptides with exquisite precision, even in crowded samples.
Simultaneously, "time focusing" empowers parallel accumulation of these separated ions, followed by rapid, sequential fragmentation. This ingenious design unlocks:
The result? Comprehensive proteome coverage, deeper insights into complex biological systems, and faster breakthroughs in your research.
PASEF® is not just a tool; it's a game-changer for biomolecular research, from peptides to proteins, to lipids and metabolites. If you demand cutting-edge proteomics solutions for superior speed, sensitivity, and selectivity, PASEF® exclusively on the timsTOF is your answer.
dda-PASEF® is a data-dependent acquisition (DDA) method that uses parallel accumulation-serial fragmentation (PASEF®) technology to improve the speed, sensitivity, and comprehensiveness of proteomics analysis.
DDA is a traditional proteomics method that selects and fragments peptides for identification and quantification based on their mass-to-charge ratio (m/z). PASEF® is a new method that uses trapped ion mobility separation (TIMS) to separate peptides based on their size, shape, and charge before fragmentation. This allows for the identification of a wider range of peptides and a more comprehensive understanding of the proteome.
Here's why dda-PASEF® stands out:
dda-PASEF® excels in:
"PASEF® already out of the box increases the precursor coverage of lipids with associated MS2 spectra to 70%. These MS2 spectra are essential for the correct identification of lipids. On top, the use of CCS adds additional confidence in annotation by adding an additional layer of information to RT, MS, and MS/MS."
dia-PASEF® is a data-independent acquisition (DIA) method that uses parallel accumulation-serial fragmentation (PASEF®) technology to attain superior speed, comprehensive and ultra-sensitive quantitative proteomics.
Here's why dia-PASEF® stands out:
dia-PASEF® shines in:
If you are looking to gain the most comprehensive data while not sacrificing quantitative specificity for your biological data, dia-PASEF® is the method for you.
"Tissue analysis is a key area in clinical proteomics, yet extremely challenging as tissue sections and biopsies comprise a vastly heterogenous cell population. The dia-PASEF® acquisition mode on the timsTOF HT instrument quantifies proteins across a large dynamic range even in notoriously difficult samples such as cardiac tissue without sacrificing throughput or sensitivity."
prm-PASEF® is a targeted proteomics method that combines the advantages of parallel reaction monitoring (PRM) and parallel accumulation-serial fragmentation (PASEF®). PRM is a specific method for the identification and quantification of known analytes in the sample, while PASEF® is a technique that increases the sensitivity and selectivity of proteomics analysis.
Here's why prm-PASEF® sets the standard:
The prm-PASEF® advantage:
If you are looking for a targeted proteomics method that offers increased sensitivity, selectivity, and multiplexing capabilities, then prm-PASEF® is the method for you.
"About a year ago, my laboratory started a collaboration with Bruker to develop the prm-PASEF® method on the timsTOF Pro. During the development of the prm-PASEF® method, we saw that the dual trapped ion mobility device could store ions and release them as very sharp, intense peaks coupled to the high-resolution TOF is a wonderful way to increase the signal and gain in intensity. Moreover, we also have been positively impressed by the instrument's reliability; it's fantastic!"
Discover just a few of the innovative ways that customers are using PASEF® to advance their research. As PASEF® technology continues to develop, we can expect to see even more innovative applications for this powerful tool.
Just a click of a button to empower you to dissect any sample.
Ready to join the PASEF® revolution? Explore its potential today.
dda-PASEF® variations
dia-PASEF® variations
dia-PASEF® with dda-like spectra
prm-PASEF® variations
PASEF® is a revolutionary mass spectrometry technology that is exclusively offered by Bruker. It is the most advanced technology available for proteomics analysis, combining the advantages of orthogonal trapped ion mobility and time-of-flight separation to deliver unprecedented levels of selectivity, speed, and sensitivity. This makes PASEF® the ideal choice for a wide range of applications, from proteome-wide analysis to single-cell analysis.
If you are looking for the most advanced mass spectrometry technology for proteomics analysis, then PASEF® is the clear choice. Contact Bruker today to learn more about PASEF® and how it can help you advance your research.
For Research Use Only. Not for use in clinical diagnostic procedures.