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Reveal tumor cell subpopulations in greater molecular detail

MALDI Guided SpatialOMx provides an excellent possibility to discover deep molecular insight into heterogeneous tumor subtype by integrating the regiospecific information of an imaging technique with the molecular depth of 4D-Omics.

In order to understand how individual cells act and interact in the context of their heterogeneous tissue environment, it is imperative to examine their molecular profiles in situ. However, traditional analysis tools are limited, either disregarding the spatial component as with homogenization followed by LC-MS, or monitoring a very limited range of proteins as with immuno-based imaging. To better understand the heterogeneous tumor environment and effect better management options why not utilize more of the molecular information available in the tissue environment?

MALDI Guided SpatialOMx allows subtyping of tissue using thousands of label-free images of compounds ranging from metabolites to proteins.  Further, each subpopulation of cells can be subsequently targeted for 4D-OMICS analysis to extract deepest molecular insight with spatial context that only SpatialOMx can provide.

Using the links below, learn more about a recent study that used lipid images to subtype breast tumor followed by 4D-Proteomic analysis of each subpopulation to revel that each subpopulation of cells expressed different proteomic activity.

Publication in Proteomics, August 7, 2020

MS Imaging‐guided Microproteomics for Spatial Omics on a Single Instrument

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Bruker Application Note:

MALDI Guided SpatialOMx uncovers proteomic profiles in tumor subpopulations of breast cancer

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Listen to the author in the following On-Demand presentation:

Next level imaging Mass Spectrometry: Single cells in focus with SpatialOMx

Prof. Ron Heeren, PhD, Maastricht University, Division Head Imaging MS, Maastricht, Netherlands

Abstract

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Whether you are probing the cellular proteome, lipidome or metabolome, Imaging provides a literal map of molecular expression in the specimen. Often, knowing where molecular expression changes can be just as important as knowing if expression changes. This can be especially true if certain compounds are highly spatially concentrated or if molecules co-distribute in specific compartments, vital information that is lost when examining only homogenized samples. SpatialOMx biomarker discovery is most complete with contextualized spatial information providing important clues into intercellular communications networks that are integral to cancer growth.

SpatialOMx® is providing context to OMICS - Learn more in this interview with Michael Easterling:

What’s one of the most important prerequisites for MALDI imaging?  LC-MS!  Find out how SpatialOMx® is providing context to OMICS and where the field is going next and why pathology researchers care in an interview with Michael L. Easterling, Manager of Bruker’s Imaging Business.

Grant Support Materials

Brochure: timsTOF Pro/fleX The new standard for shotgun proteomics
Four reasons to switch to 4D-Proteomics™ on the timsTOF platform. To provide concise arguments for replacement of older 3D mass spectrometers with the 4D capable, timsTOF platform and its unique TIMS (Trapped Ion Mobility Spectrometry) capability which adds the CCS dimension to retention time, precursor mass, and MS/MS.

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Brochure: Guide for writing successful proposals for SpatialOMx and timsTOF fleX
 
MALDI Guided SpatialOMx® represents a quantum advance in how tissue is analyzed. It combines a label-free imaging technique that delivers sensitive distribution mapping for both targeted and untargeted compounds, ranging from metabolites to lipids to proteins with regionally targeted 4D-Omics analysis.

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You want more Insights into SpatialOMx? Listen to our SpatialOMx experts in Bruker's On-Demand webinars:

SpatialOMx – bringing context to proteomics through molecular pathology

Dr. Shannon Cornett, MALDI Imaging Business Unit, Bruker Daltonics

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Imaging pathways: SpatialOMx captures small molecule and lipid networks across tissues to bridge metabolomics and disease

Dr. Shannon Cornett, MALDI Imaging Business Unit, Bruker Daltonics

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In situ image identification using MALDI

Dr. Janina Oetjen, Application Specialist MALDI Imaging, Bruker Daltonics

View Recording

 

 

For further information, please contact:

Sales:
ms.sales.bdal@bruker.com to arrange for a demo or to contact sales

Marketing:
Nancy Wright-Ross, Senior Marketing Manager
Nancy.wright-ross@bruker.com

 

For Research Use Only. Not for use in clinical diagnostic procedures.