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Reveal tumor cell subpopulations in greater molecular detail

MALDI Guided SpatialOMx provides an excellent possibility to discover deep molecular insight into heterogeneous tumor subtype by integrating the regiospecific information of an imaging technique with the molecular depth of 4D-Omics.

In order to understand how individual cells act and interact in the context of their heterogeneous tissue environment, it is imperative to examine their molecular profiles in situ. However, traditional analysis tools are limited, either disregarding the spatial component as with homogenization followed by LC-MS, or monitoring a very limited range of proteins as with immuno-based imaging. To better understand the heterogeneous tumor environment and effect better management options why not utilize more of the molecular information available in the tissue environment?

MALDI Guided SpatialOMx allows subtyping of tissue using thousands of label-free images of compounds ranging from metabolites to proteins.  Further, each subpopulation of cells can be subsequently targeted for 4D-OMICS analysis to extract deepest molecular insight with spatial context that only SpatialOMx can provide.

Using the links below, learn more about a recent study that used lipid images to subtype breast tumor followed by 4D-Proteomic analysis of each subpopulation to revel that each subpopulation of cells expressed different proteomic activity.

Publication in Proteomics, August 7, 2020

MS Imaging‐guided Microproteomics for Spatial Omics on a Single Instrument

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Bruker Application Note:

MALDI Guided SpatialOMx uncovers proteomic profiles in tumor subpopulations of breast cancer

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Listen to the author in the following On-Demand presentation:

Next level imaging Mass Spectrometry: Single cells in focus with SpatialOMx

Prof. Ron Heeren, PhD, Maastricht University, Division Head Imaging MS, Maastricht, Netherlands

Abstract

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Whether you are probing the cellular proteome, lipidome or metabolome, Imaging provides a literal map of molecular expression in the specimen. Often, knowing where molecular expression changes can be just as important as knowing if expression changes. This can be especially true if certain compounds are highly spatially concentrated or if molecules co-distribute in specific compartments, vital information that is lost when examining only homogenized samples. SpatialOMx biomarker discovery is most complete with contextualized spatial information providing important clues into intercellular communications networks that are integral to cancer growth.

You want more Insights into SpatialOMx? Listen to our SpatialOMx experts in Bruker's On-Demand webinars:

SpatialOMx – bringing context to proteomics through molecular pathology

Dr. Shannon Cornett, MALDI Imaging Business Unit, Bruker Daltonics

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Imaging pathways: SpatialOMx captures small molecule and lipid networks across tissues to bridge metabolomics and disease

Dr. Shannon Cornett, MALDI Imaging Business Unit, Bruker Daltonics

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In situ image identification using MALDI

Dr. Janina Oetjen, Application Specialist MALDI Imaging, Bruker Daltonics

View Recording

 

 

For further information, please contact:

Sales:
ms.sales.bdal@bruker.com to arrange for a demo or to contact sales

Marketing:
Nancy Wright-Ross, Senior Marketing Manager
Nancy.wright-ross@bruker.com

 

For Research Use Only. Not for use in clinical diagnostic procedures.