On-Demand Sessions: Enabling Light Sheet Imaging and Tissue Clearing in a Shared Facility
Learn how our guest speaker uses different imaging techniques in an imaging facility
Enabling Light Sheet Imaging and Tissue Clearing in a Shared Facility
PRESENTATION HIGHLIGHTS:
- [00:01:03] Overview of CCHMC foundation and facility
- [00:03:43] Challenges of operating a facility in a large academic medical center with a diverse user base
- [00:06:39] Tissue clearing for light sheet imaging with Bruker's LCS SPIM
- [00:27:14] Image and sample processing to improve data analysis
- [00:38:34] Conclusion
Q&A
PRESENTATION HIGHLIGHTS:
- [00:00:00] Would expansion methods make LCS usable for subcellular resolution?
- [00:02:31] Has light-sheet replaced traditional sectioning workflows for some of your users?
- [00:04:10] Have you had any luck with imaging a cleared sample on the LCS and then doing a reverse clearing protocol, and then bringing that same sample to confocal for sub-cellular analysis of the same tissue?
- [00:05:18] To what extent do you see AI tools being integrated into workflows? Do you see them replacing manual cell counting?
