Cleared Sample Imaging

Combining tissue clearing and state-of-the-art imaging, to visualize otherwise invisible structures

Cleared-Sample Imaging Solutions

Bruker Luxendo light-sheet microscopes are optimized for 3D imaging of intricate tissue structures and deliver best-in-class 3D imaging of cleared samples. The variety of Bruker Luxendo Selective-Plane Illumination Microscopes (SPIMs) allows researchers to choose the best microscope for their sample, depending on mounting, sample size, and optics. These characteristics are particularly important for complex studies such as brain connectomics or tumor metastasis.

 

Tissue clearing in combination with light-sheet microscopy has become the go-to technique to visualize large, and by default, opaque tissues. Thanks to technological advancements, large structures can be imaged within minutes, providing new insights in the fields of neuroscience and developmental biology. Furthermore, as tissue clearing is a rapidly evolving field, Bruker Luxendo SPIMs are compatible with all clearing solutions available.

Cleared Sample Imaging Gallery

For more information about cleared sample imaging with light sheet fluorescence microscopy, visit:

Neurons of a transgenic mouse expressing fluorescent protein YFP. Brain was cleared with Clarity.

Courtesy of:
Dr. Zhang Dan, Tsinghuan University, China

Cleared Mouse Lymph Node
MuVi SPIM

Cleared mouse lymph node. High endothelial venules (642 nm, red) and autofluorescence (488 nm, green) to visualize surrounding tissue. Imaged on the MuVi SPIM.

Courtesy of:
Jens Stein, University of Bern, Bern, Switzerland

Cleared Mouse Lymph Node
QuVi SPIM

BABB-cleared mouse lymph node. High endothelial venules (red) and autofluorescence (green) to visualize surrounding tissue.

Courtesy of:
Jens Stein, University of Bern, Bern, Switzerland

Light-sheet images of a lung taken with the LCS SPIM

Courtesy of:
Ayelen Melina, Santamans Recchini, and Gaudalupe Sabio Buzo, Stress kinases in Diabetes, Cancer and Cardiovascular Disease Laboratory and Unit of Microscopy and Dynamic Imaging, Centro Nacional de Investigaciones Cardiovasculares Carlos III (CNIC), Madrid.

Cleared Mouse Head

Labeled with anti-tuj1 (green) to mark developing nerves and with anti-desmin (red) to mark differentiating muscles. Tiled image (6 × 5).
Imaged on the MuVi SPIM.

Courtesy of:
Glenda Comai, Institut Pasteur, Paris, France

Developing Nerves in a Whole Mouse Embryo

Developing nerves in a whole mouse embryo.
Sample cleared with DBE. Tiled image (3 x 4) acquisition. Scalebars: 1mm.
Imaged on the LCS SPIM.

Sample courtesy of:
James Muller, MSKCC, New York, USA

Neuronal Network

Neuronal network (stained with GFP) of a CUBIC-cleared mouse brain

Color-coded depth representation: maximal depth displayed 1.23 mm.
Imaged on the MuVi SPIM.

Courtesy of:
Montserrat Coll Lladó, European Molecular Biology Laboratory (EMBL), Barcelona, Spain

Contact Us

Input value is invalid.

* Please fill out the mandatory fields.

Please enter your first name
Please enter your last name
Please enter your e-mail address
Please enter a valid phone number
Please enter your Company/Institution
What best describes your current interest?
Please add me to your email subscription list so I can receive webinar invitations, product announcements and events near me.
Please accept the Terms and Conditions