Cleared Sample Imaging
Cleared-Sample / Cleared-Tissue Applications
The 3D imaging capabilities of light-sheet microscopes, in combination with optical clearing techniques, enable visualization of very large, cleared specimens in-toto, comprehensive and non-destructive within minutes. It has thus become a valuable tool for 3D microstructure analysis of tissues (e.g. in neuroscience, developmental biology, and connectomics).
Bruker light-sheet microscopes are optimized for 3D imaging of intricate tissue structures and deliver best-in-class 3D imaging of cleared samples. These capabilities are particularly relevant to studying the brain or the central nervous tissue in neuroscience, analyzing organ development, or investigating tumor structure and genesis in oncology.
Cleared Sample Imaging Gallery
For more information about cleared sample imaging with light sheet fluorescence microscopy, visit:
Cleared Mouse Lymph Node
MuVi SPIM
Cleared mouse lymph node. High endothelial venules (642 nm, red) and autofluorescence (488 nm, green) to visualize surrounding tissue. Imaged on the MuVi SPIM.
Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland
Cleared Mouse Lymph Node
QuVi SPIM
BABB-cleared mouse lymph node. High endothelial venules (red) and autofluorescence (green) to visualize surrounding tissue. Imaged on the QuVi SPIM.
Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland
Cleared Mouse Head
Labeled with anti-tuj1 (green) to mark developing nerves and with anti-desmin (red) to mark differentiating muscles. Tiled image (6 × 5).
Imaged on the MuVi SPIM.
Courtesy of:
Glenda Comai
Institut Pasteur
Paris, France
Transgenic Drosophila Line Expressing UAS-mCD8-GFP with the Mef2-GAL4 Driver Line
The fruit fly was optically cleared and RI adjusted using alcohol dilutions and ECI. Size: 1.7 mm length (head to tail).
Imaged on the LCS SPIM.
Courtesy of:
Susanne Önel
Philipps University Marburg
Marburg, Germany
Neuronal Network
Neuronal network (stained with GFP) of a CUBIC-cleared mouse brain
Color-coded depth representation: maximal depth displayed 1.23 mm.
Imaged on the MuVi SPIM.
Courtesy of:
Montserrat Coll Lladó
European Molecular Biology Laboratory (EMBL)
Barcelona, Spain
Developing Nerves in a Whole Mouse Embryo
Developing nerves in a whole mouse embryo.
Sample cleared with DBE. Tiled image (3 x 4) acquisition. Scalebars: 1mm.
Imaged on the LCS SPIM.
Sample courtesy of:
James Muller
MSKCC
New York, USA
