Cleared Sample Imaging

Modifying the properties of usually opaque samples to render them transparent while keeping their structure intact

Применения

Cleared-Sample / Cleared-Tissue Applications

The 3D imaging capabilities of light-sheet microscopes, in combination with optical clearing techniques, enable visualization of very large, cleared specimens in-toto, comprehensive and non-destructive within minutes. It has thus become a valuable tool for 3D microstructure analysis of tissues (e.g. in neuroscience, developmental biology and connectomics).
The MuVi SPIM CS and the LCS SPIM are optimized for 3D imaging of intricate tissue structures, relevant to studying the brain or the central nervous tissue in neuroscience, to analyzing organ development or to investigating tumor structure and genesis in oncology. They provide innovative solutions in sample mounting, sample size and optics for best-in-class 3D imaging of cleared samples. While the MuVi SPIM CS enables imaging at subcellular resolution and samples sizes up to whole mouse brains, the LCS SPIM is optimized for very large samples up to entire adult mice realizing visualization of complete neuronal or vasculature networks at cellular resolution.

Cleared Mouse Lymph Node
MuVi SPIM CS

Cleared mouse lymph node. High endothelial venules (642 nm, red) and autofluorescence (488 nm, green) to visualize surrounding tissue. Imaged on the MuVi SPIM CS.

Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland

Cleared Mouse Lymph Node
QuVi SPIM

BABB-cleared mouse lymph node. High endothelial venules (red) and autofluorescence (green) to visualize surrounding tissue. Imaged on the QuVi SPIM.

Courtesy of:
Jens Stein
University of Bern
Bern, Switzerland

Cleared Mouse Head

Labeled with anti-tuj1 (green) to mark developing nerves and with anti-desmin (red) to mark differentiating muscles. Tiled image (6 × 5).
Imaged on the MuVi SPIM CS.

Courtesy of:
Glenda Comai
Institut Pasteur
Paris, France

Transgenic Drosophila Line Expressing UAS-mCD8-GFP with the Mef2-GAL4 Driver Line

The fruit fly was optically cleared and RI adjusted using alcohol dilutions and ECI. Size: 1.7 mm length (head to tail).
Imaged on the LCS SPIM.

Courtesy of:
Susanne Önel
Philipps University Marburg
Marburg, Germany

Neuronal Network

Neuronal network (stained with GFP) of a CUBIC-cleared mouse brain

Color-coded depth representation: maximal depth displayed 1.23 mm.
Imaged on the MuVi SPIM CS.

Courtesy of:
Montserrat Coll Lladó
European Molecular Biology Laboratory (EMBL)
Barcelona, Spain

Developing Nerves in a Whole Mouse Embryo

Developing nerves in a whole mouse embryo.
Sample cleared with DBE. Tiled image (3 x 4) acquisition. Scalebars: 1mm.
Imaged on the LCS SPIM.

Sample courtesy of:
James Muller
MSKCC
New York, USA

Contact Us

Contact Us

* Please fill out the mandatory fields.

Please enter your first name
Please enter your last name
Please enter your e-mail address
Please enter a valid phone number
Please enter your Company/Institution
What best describes your current interest?
Please add me to your email subscription list so I can receive webinar invitations, product announcements and events near me.
Please accept the Terms and Conditions

Этот сайт защищен reCAPTCHA, и применяются Политика конфиденциальности и и Условия использования Google.