Bruker's Multi-View Selective-Plane Illumination Microscope (SPIM) is not only the fastest light-sheet system on the market, but also incorporates years of Luxendo imaging experience and innovation. Due to its modular concept, MuVi SPIM facilitates both live-sample (LS) and cleared-sample (CS) imaging by an acquisition unit exchange.
MuVi SPIM allows for high-speed volumetric acquisition of dynamic processes in live specimens, as well as cleared sample imaging with compatibility with any clearing method.
MuVi SPIM features a unique 4-axis concept and is available in various configurations to enable:
The MuVi SPIM is equipped with a motorized sample holder which enables sample rotation by 360°. Although sample rotation is not required to achieve in-toto imaging, a single 90° rotation of the sample can provide isotropic resolution and improve image quality. This is at least four times faster compared to conventional SPIM.
Built on Bruker's history of innovation, our next-generation MuVi SPIM incorporates several new features to enhance image quality, shorten acquisition speed, and allow high-resolution and artifact-free imaging of a broader range of samples.
When imaging, obstacles like pigments or cell nuclei can interfere with light, leading to images with shadows and striping artifacts. The next-generation SPIM employs dual de-striping via pivot scanning which generates a homogenous illumination profile without compromising acquisition speed.
When imaging thicker samples or opaque specimens, light scattering can lead to reduced contrast and image blur. MuVi SPIM has implemented an elegant approach to enhance image contrast by offering:
When imaging, obstacles like pigments or cell nuclei can interfere with light, leading to images with shadows and striping artifacts. The next-generation SPIM employs dual de-striping via pivot scanning which generates a homogenous illumination profile without compromising acquisition speed.
Thanks to state-of-the-art technologies, ultra-fast varifocal lenses can be used to fine-tune the system’s optics. Luxendo SPIMs use devices that are driven by an acoustic wave, called tunable acoustic gradient lenses (or TAG lenses), for adaptive optics. These TAG lenses allow better light-sheet control and uniformity while reducing axial stretching.
The sample chamber and all objective lenses are mounted on the octagon, the optical core unit that holds all the objective lenses.
Different octagons can support different magnifications and objective NAs and are compatible with objectives optimized for either live or cleared sample imaging.
The octagon can be easily removed and replaced while maintaining its optical alignment.
Equipped with a full environmental control module, MuVi SPIM supports imaging of various live specimens over hours and days without altering their biology. By enabling precise control over temperature (20-37 °C), gas concentration (CO2, O2, N2), and humidity, MuVi SPIM delivers unique advantages for research in the fields of:
Taking full advantage of light-sheet technology, imaging speed is significantly greater than with confocal microscopy. With excellent speed and low phototoxicity, samples are imaged gently and in-depth. This is particularly useful for fast biological dynamics, such as calcium imaging or blood flow.
The ability to spatiotemporally control, manipulate, and alter biological processes is key to revealing relevant mechanisms at play. A photomanipulation (PM) module can be added to MuVi SPIM, allowing for experiments such as photoablation, cauterization, optogenetics, and fluorescence recovery after photobleaching (FRAP) with:
Used in combination with optical clearing techniques, MuVi SPIM enables comprehensive and non-destructive visualization of very large, in-toto cleared specimens within minutes. This is particularly useful for studying:
MuVi SPIM can image a broad range of cleared samples with different sizes, ranging from 3D organoids to large tissue samples and up to the size of a cleared mouse brain. This is accomplished by:
MuVi SPIM provides an innovative solution for sample mounting, minimizing users' exposure to clearing reagents. This is accomplished by:
An important goal in microscopy is sample health and integrity. To support this, MuVi SPIM is equipped with environmental control that supports long-term imaging (hours to days). In addition to a generous sample chamber for immersion medium, this includes control over:
Detection Objective | Effective Magnification | Pixel size (nm) | Field of View (µm)* |
---|---|---|---|
20x 1.0 NA | 11.1x | 585 | 1198 |
22.2x | 293 | 599 | |
33.3x | 195 | 399 | |
44.4x | 146 | 299 | |
16x 0.8 NA | 8x | 812 | 1664 |
16x | 406 | 832 | |
24x | 271 | 554 | |
32x | 203 | 416 |
Detection Objective | Effective Magnification | Pixel size (nm) | Field of View (µm)* |
---|---|---|---|
20x 1.0 NA | 10x | 650 | 1331 |
20x | 325 | 665 | |
30x | 217 | 444 | |
40x | 163 | 333 | |
10x 0.5 NA | 5x | 1300 | 2662 |
10x | 650 | 1331 | |
15x | 433 | 887 | |
20x | 325 | 666 | |
4x 0.28 NA | 2.2x | 2925 | 5990 |
4.4x | 1463 | 2995 | |
6.7x | 975 | 1997 | |
8.9x | 731 | 1497 |
Bruker's LuxBundle software saves time and enhances productivity by providing:
MuVi SPIM records a sample from different angles/views and generates images composed of multiple tiles. The LuxBundle software ensures high-quality, 360° crisp images of the sample that compensate for absorption and scattering. Features include:
LuxBundle's integrated 3D data viewer allows researchers to inspect the entire dataset directly after acquisition. This gives users control over their data with key capabilities, including: