Light-Sheet Microscopes

MuVi SPIM

Superior multiview imaging for live and cleared samples

Destaques

MuVi SPIM

Bruker's MuVi Selective-Plane Illumination Microscope (SPIM) is the fastest multi-angle view system on the market. MuVi incorporates years of Luxendo light-sheet technology innovations to provide high-speed volumetric acquisition for analyzing dynamic processes in delicate live specimens, as well as offering compatibility with any clearing method. 

 

With its highly modular concept, the MuVi SPIM facilitates either live-sample (LS) or cleared-sample (CS) imaging simply by exchanging the acquisition unit. A variety of other advanced hardware and software integrations can be easily configured — often in the field — to support unique and evolving multidimensional experimental needs.

Best-in-class
multiview imaging capabilities
Capture fast biological processes and high-quality volumetric data with minimal striping artifacts.
Advanced
hardware and software integrations
Allow precise configuration and easy adjustment to meet current and future experimental requirements.
Multimodal
modular concept
Facilitates live and cleared sample imaging with easily interchangeable acquisition units (octagons).

Características

Features

Best-in-Class Multiview Imaging Capabilities

Convenient Multiview Imaging

MuVi SPIM features a unique 4-axis concept and is available in various configurations to enable:

  • Simultaneous illumination and detection from two opposing directions without rotation
  • Unparalleled acquisition speed
  • Free rotation axis for optimal sample positioning and 360° view
  • Long-term stability for imaging time series over days

Isotropic Resolution with 360° Illumination and Detection

The MuVi SPIM is equipped with a motorized sample holder which enables sample rotation by 360°. Although sample rotation is not required to achieve in-toto imaging, a single 90° rotation of the sample can provide isotropic resolution and improve image quality. This is at least four times faster compared to conventional SPIM.

     

Zebrafish expressing H2A::GFP acquired simultaneously from two opposing directions (left, right). Data fusion of the two views yields a high quality in-toto image of the sample (middle).

Next-Generation Technology

Built on Bruker's history of innovation, our next-generation MuVi SPIM incorporates several new features to enhance image quality, shorten acquisition speed, and allow high-resolution and artifact-free imaging of a broader range of samples.

New Advanced Features

Larger Field-of-View (FOV) and Superior Axial Resolution

MuVi SPIM obtains large FOV images at high 3D spatial resolution by using a tightly focused Gaussian beam swept at a high speed along the illumination axis. Integration over time and space leads to an elongated, uniform, and thin light-sheet to provide uniform axial resolution over large fields of view.

Improved Signal-to-Noise

When imaging thicker samples or opaque specimens, light scattering can lead to reduced contrast and image blur. MuVi SPIM has implemented an elegant approach to enhance image contrast by offering:

  • Scanned light-sheet
  • Adjustable light-sheet thickness
  • Robust aberration tolerance (even in complex samples)
  • Line illumination for improved background suppression

Impressive Elimination of Striping Artifacts

When imaging live, cleared, or fixed biological samples, obstacles like pigments or cell nuclei can absorb or scatter light, leading to images with shadows and striping artifacts. The next-generation MuVi SPIM employs a dual de-striping via pivot scanning, which generates a homogenous illumination profile without compromising acquisition speed. 

Flexible Live and Cleared Sample Imaging Options

Unique Multimodal Concept

MuVi SPIM's highly modular multimodal concept allows researchers to choose the ideal configuration and capabilities needed to support their specific experimental needs. Most notably, it facilitates imaging of either live or cleared samples simply by switching the acquisition unit (octagons). These and other modules can be easily added or exchanged if experimental parameters or imaging requirements change. This provides unique advantages for ongoing and innovative research, as well as its use in multi-user environments.

MuVi SPIM LS

Optimized for live, long-term imaging with full environmental control and an optional photomanipulation module

MuVi SPIM CS

Optimized for cleared samples and supports any kind of clearing solution to maintain optimal image quality

Transition between live sample and cleared sample system configurations in just minutes.

The sample chamber and all objective lenses are mounted on the octagon, the optical core unit that holds all the objective lenses.

Different octagons can support different magnifications and objective NAs and are compatible with objectives optimized for either live or cleared sample imaging.

The octagon can be easily removed and replaced while maintaining its optical alignment.

Octagon Exchange from Cleared Sample to Live Sample Imaging: The video shows the fast and easy transition from Cleared Sample (CS) to Live Sample (LS) imaging with the light-sheet microscope MuVi SPIM from Luxendo.
Octagon Exchange from Live Sample to Cleared Sample Imaging: The video shows the fast and easy transition from Live sample (LS) to Cleared Sample imaging with the light-sheet microscope MuVi SPIM from Luxendo.

Aplicações

Applications

Pure Live Imaging

Native and Natural Environment

Equipped with a full environmental control module, MuVi SPIM supports imaging of various live specimens over hours and days without altering their biology. By enabling precise control over temperature (20-37 °C), gas concentration (CO2, O2, N2), and humidity, MuVi SPIM delivers unique advantages for research in the fields of:

  • Developmental biology
  • Marine biology
  • Neurobiology and neurodevelopment
  • Oncology

Unparalleled Imaging Speed for in-toto Imaging

With its exceptional imaging speed, the MuVi SPIM can capture many dynamic biological events. This makes it:

  • Four times faster than single-view light-sheet
  • One hundred to a thousand times faster than confocal microscopy
  • Ideal for functional imaging
  • Perfect for cellular and subcellular tracking


Advantageous Photomanipulation

The ability to spatiotemporally control, manipulate, and alter biological processes is key to revealing relevant mechanisms or mechano-chemical interplay. A photomanipulation (PM) module can be added to MuVi SPIM, which allows for advanced experiments in photoablation, FRAP, optogenetics, and cauterization with:

  • Beam coupled into detection objective
  • Diffraction-limited illumination spot
  • Flexible ROI generation (point, straight line, freeform, and square)
  • Laser from CW UV/VIS to pulsed IR

MuVi SPIM LS


     

Cell tracking in drosophila embryo development created with arivis Vision4D 3D visualization and analysis software. Imaged on the MuVi SPIM (Courtesy of: Celia Smits and Stanislav Y. Shvartsman, Department of Molecular Biology, Princeton University)
Zebrafish imaged on the MuVi SPIM. Stitched from 5 stacks in Imaris, each 340 slices, 16 hour/10min. Fish growth can be observed (Courtesy of: Prof. Jingxia Liu Huazhong Agricultural University, China)

Comprehensive Cleared-Sample Imaging

Advanced Cleared-Sample Applications

Used in combination with optical clearing techniques, MuVi SPIM enables comprehensive and non-destructive visualization of very large, in-toto cleared specimens within minutes. This is particularly useful for studying:

  • 3D microstructure analysis of tissues
  • Brain and central nervous system tissues
  • Organ development
  • Tumorigenesis

High Flexibility of Sample Size and Clearing Method

MuVi SPIM can image a broad range of cleared samples with different sizes, ranging from 3D organoids to large tissue samples and up to the size of a cleared mouse brain. This is accomplished by:

  • Innovative optical design compatible with any kind of clearing solution
  • Different configurations of detection lenses to match the properties of your samples
  • 10x10 mm area accessible for imaging

Innovative and Easy Sample Handling

MuVi SPIM provides an innovative solution for sample mounting, minimizing users' exposure to clearing reagents. This is accomplished by:

  • Overhead 3D translation/rotation unit for sample mounting
  • Support of various mounting methods (e.g. hook, plate, pin, cuvette, etc.)
  • Fast objective exchange for easy transition between live-sample (LS) and cleared-sample (CS) imaging
  • Easy maintenance and cleaning of objectives and chamber

MuVi SPIM CS


     

Visualization of complete neuronal network of whole cleared brain imaged on Luxendo MuVi SPIM CS. Neurons of a transgenic mouse expressed fluorescent protein YFP and the brain was cleared with Clarity (Courtesy: Dr. Zhang Dan, Tsinghuan University, China)
Antibody labeled supporting cells of guineapig cochlea cleared with Cubic and acquired on MuVi SPIM equipped with the Cleared Sample acquisition unit. Visualization of the cochlea enables investigation of mechanisms leading to hearing loss (Courtesy: Prof. Löwenheim, Uniklinikum Tübingen)

Especificações

Specifications

MuVi LS

Illuminations Detection Effective Magnification Field of View (µm) Pixel Size (nm) Optical Resolution (nm)
10x / 0.3 NA Olympus 20x / 1.0 NA 16.7x 800 390 300
    22.2x 600 292  
    33.3x 400 195  
    44.4x 300 146  
10x / 0.3 NA Nikon 16x / 0.8 NA 12x 1100 542 375
    16x 830 406  
    24x 550 271  
    32x 415 203  

MuVi CS

Illuminations Detection Effective Magnification Field of View (µm) Pixel Size (nm) Optical Resolution (nm)
10x / 0.3 NA Nikon 20x / 1.0 NA 15x 890 434 300
    20x 665 325  
    30x 445 217  
    40x 333 162  
10x / 0.3 NA Nikon 10x / 0.5 NA 7.5x 1775 867 600
    10x 1330 650  
    15x 890 434  
    20x 665 325  
4x / 0.13 NA Olympus 4x / 0.28 NA 2.2x 6600 2960 1100
    4.4x 3300 1480  
    6.6x 1996 980  
    8.8x 1515 740  

 

Software

Software

All-in-One LuxControl Software

Intuitive design

Bruker's LuxControl software saves time and enhances productivity by providing:

  • All-in-one, easy-to-use interface for acquisition, viewing, and post-processing
  • Fully scriptable microscope control and post-processing via open interface (e.g., Python or any other language) ready for custom "smart" microscopy
  • High reproducibility of experiments: all parameters are saved in the metadata and configurations can be saved for future experiments
  • Data formats (.tiff, .hdf5, .ims) compatible with common image processing software: Imaris, Aivia, BigDataViewer, Arivis, Fiji, Python, Matlab, Napari

Impressive Image Post Processor

MuVi SPIM records a sample from different angles/views and generates images composed of multiple tiles. The LuxControl software ensures high-quality, 360° crisp images of the sample that compensate for absorption and scattering. Features include:

  • Multi-color alignment
  • Tile stitching of hundreds of tiles for large samples
  • Multi-view image fusion and deconvolution

3D Data Viewer

LuxControl's integrated 3D data viewer allows researchers to inspect the entire dataset directly after acquisition. This gives users control over their data with key capabilities, including:

  • The ability to turn tile stitching on or off
  • Both raw and post-processed images
  • Fast viewing of multi-terabyte data sets
  • Flexible options to draw and annotate regions and landmarks
Cleared mouse embryo labeled with methylene blue (cyan) and showing autofluorescence (magenta). Image composed of 12 tiles and 920 planes, all processed and stitched with LuxControl (Image courtesy of Montserrat Coll Lladó, European Molecular Biology Laboratory, Bercelona, Spain)

Webinars

Contate o especialista

Contact Us

* Please fill out the mandatory fields.

Please enter your first name
Please enter your last name
Please enter your e-mail address
Please enter a valid phone number
Please enter your Company/Institution
What best describes your current interest?
Please add me to your email subscription list so I can receive webinar invitations, product announcements and events near me.
Please accept the Terms and Conditions

Este site é protegido pela reCAPTCHA e pelo Google Política de Privacidade e Termos de Serviço do Google.