MuVi SPIM
하이라이트
MuVi SPIM
Bruker's MuVi Selective-Plane Illumination Microscope (SPIM) is the fastest multiview system on the market. MuVi incorporates years of Luxendo light-sheet technology innovations to provide high-speed volumetric acquisition for analyzing dynamic processes in delicate live specimens, as well as offering compatibility with any clearing method.
With its highly modular concept, the MuVi SPIM facilitates either live-sample (LS) or cleared-sample (CS) imaging simply by exchanging the acquisition unit. A variety of other advanced hardware and software integrations can be easily configured — often in the field — to support unique and evolving multidimensional experimental needs.
특징
Best-in-Class Multiview Imaging Capabilities
Convenient Multiview Imaging
MuVi SPIM features a unique 4-axis concept and is available in various configurations to enable:
- Simultaneous illumination and detection from two opposing directions without rotation
- Unparalleled acquisition speed
- Free rotation axis for optimal sample positioning and 360° view
- Long-term stability for imaging time series over days
Isotropic Resolution with 360° Illumination and Detection
The MuVi SPIM is equipped with a motorized sample holder which enables sample rotation by 360°. Although sample rotation is not required to achieve in-toto imaging, a single 90° rotation of the sample can provide isotropic resolution and improve image quality. This is at least four times faster compared to conventional SPIM.
Next-Generation Technology
Built on Bruker's history of innovation, our next-generation MuVi SPIM incorporates several new features to enhance image quality, shorten acquisition speed, and allow high-resolution and artifact-free imaging of a broader range of samples.
New Advanced Features
Larger Field-of-View (FOV) and Superior Axial Resolution
MuVi SPIM obtains large FOV images at high 3D spatial resolution by using a tightly focused Gaussian beam swept at a high speed along the illumination axis. Integration over time and space leads to an elongated, uniform, and thin light-sheet to provide uniform axial resolution over large fields of view.
Improved Signal-to-Noise
When imaging thicker samples or opaque specimens, light scattering can lead to reduced contrast and image blur. MuVi SPIM has implemented an elegant approach to enhance image contrast by offering:
- Scanned light-sheet
- Adjustable light-sheet thickness
- Robust aberration tolerance (even in complex samples)
- Line illumination for improved background suppression
Impressive Elimination of Striping Artifacts
When imaging live, cleared, or fixed biological samples, obstacles like pigments or cell nuclei can absorb or scatter light, leading to images with shadows and striping artifacts. The next-generation MuVi SPIM employs a dual de-striping via pivot scanning, which generates a homogenous illumination profile without compromising acquisition speed.
Transition between live sample and cleared sample system configurations in just minutes.
The sample chamber and all objective lenses are mounted on the octagon, the optical core unit that holds all the objective lenses.
Different octagons can support different magnifications and objective NAs and are compatible with objectives optimized for either live or cleared sample imaging.
The octagon can be easily removed and replaced while maintaining its optical alignment.
응용
Pure Live Imaging
Native and Natural Environment
Equipped with a full environmental control module, MuVi SPIM supports imaging of various live specimens over hours and days without altering their biology. By enabling precise control over temperature (20-37 °C), gas concentration (CO2, O2, N2), and humidity, MuVi SPIM delivers unique advantages for research in the fields of:
- Developmental biology
- Marine biology
- Neurobiology and neurodevelopment
- Oncology
Unparalleled Imaging Speed for in-toto Imaging
With its exceptional imaging speed, the MuVi SPIM can capture many dynamic biological events. This makes it:
- Four times faster than single-view light-sheet
- One hundred to a thousand times faster than confocal microscopy
- Ideal for functional imaging
- Perfect for cellular and subcellular tracking
Advantageous Photomanipulation
The ability to spatiotemporally control, manipulate, and alter biological processes is key to revealing relevant mechanisms or mechano-chemical interplay. A photomanipulation (PM) module can be added to MuVi SPIM, which allows for advanced experiments in photoablation, FRAP, optogenetics, and cauterization with:
- Beam coupled into detection objective
- Diffraction-limited illumination spot
- Flexible ROI generation (point, straight line, freeform, and square)
- Laser from CW UV/VIS to pulsed IR
Comprehensive Cleared-Sample Imaging
Advanced Cleared-Sample Applications
Used in combination with optical clearing techniques, MuVi SPIM enables comprehensive and non-destructive visualization of very large, in-toto cleared specimens within minutes. This is particularly useful for studying:
- 3D microstructure analysis of tissues
- Brain and central nervous system tissues
- Organ development
- Tumorigenesis
High Flexibility of Sample Size and Clearing Method
MuVi SPIM can image a broad range of cleared samples with different sizes, ranging from 3D organoids to large tissue samples and up to the size of a cleared mouse brain. This is accomplished by:
- Innovative optical design compatible with any kind of clearing solution
- Different configurations of detection lenses to match the properties of your samples
- 10x10 mm area accessible for imaging
Innovative and Easy Sample Handling
MuVi SPIM provides an innovative solution for sample mounting, minimizing users' exposure to clearing reagents. This is accomplished by:
- Overhead 3D translation/rotation unit for sample mounting
- Support of various mounting methods (e.g. hook, plate, pin, cuvette, etc.)
- Fast objective exchange for easy transition between live-sample (LS) and cleared-sample (CS) imaging
- Easy maintenance and cleaning of objectives and chamber
사양
MuVi LS
| Illuminations | Detection | Effective Magnification | Field of View (µm) | Pixel Size (nm) | Optical Resolution (nm) |
|---|---|---|---|---|---|
| 10x / 0.3 NA | Olympus 20x / 1.0 NA | 16.7x | 800 | 390 | 300 |
| 22.2x | 600 | 292 | |||
| 33.3x | 400 | 195 | |||
| 44.4x | 300 | 146 | |||
| 10x / 0.3 NA | Nikon 16x / 0.8 NA | 12x | 1100 | 542 | 375 |
| 16x | 830 | 406 | |||
| 24x | 550 | 271 | |||
| 32x | 415 | 203 |
MuVi CS
| Illuminations | Detection | Effective Magnification | Field of View (µm) | Pixel Size (nm) | Optical Resolution (nm) |
|---|---|---|---|---|---|
| 10x / 0.3 NA | Nikon 20x / 1.0 NA | 15x | 890 | 434 | 300 |
| 20x | 665 | 325 | |||
| 30x | 445 | 217 | |||
| 40x | 333 | 162 | |||
| 10x / 0.3 NA | Nikon 10x / 0.5 NA | 7.5x | 1775 | 867 | 600 |
| 10x | 1330 | 650 | |||
| 15x | 890 | 434 | |||
| 20x | 665 | 325 | |||
| 4x / 0.13 NA | Olympus 4x / 0.28 NA | 2.2x | 6600 | 2960 | 1100 |
| 4.4x | 3300 | 1480 | |||
| 6.6x | 1996 | 980 | |||
| 8.8x | 1515 | 740 |
소프트웨어
All-in-One LuxControl Software
Intuitive design
Bruker's LuxControl software saves time and enhances productivity by providing:
- All-in-one, easy-to-use interface for acquisition, viewing, and post-processing
- Fully scriptable microscope control and post-processing via open interface (e.g., Python or any other language) ready for custom "smart" microscopy
- High reproducibility of experiments: all parameters are saved in the metadata and configurations can be saved for future experiments
- Data formats (.tiff, .hdf5, .ims) compatible with common image processing software: Imaris, Aivia, BigDataViewer, Arivis, Fiji, Python, Matlab, Napari
Impressive Image Post Processor
MuVi SPIM records a sample from different angles/views and generates images composed of multiple tiles. The LuxControl software ensures high-quality, 360° crisp images of the sample that compensate for absorption and scattering. Features include:
- Multi-color alignment
- Tile stitching of hundreds of tiles for large samples
- Multi-view image fusion and deconvolution
3D Data Viewer
LuxControl's integrated 3D data viewer allows researchers to inspect the entire dataset directly after acquisition. This gives users control over their data with key capabilities, including:
- The ability to turn tile stitching on or off
- Both raw and post-processed images
- Fast viewing of multi-terabyte data sets
- Flexible options to draw and annotate regions and landmarks
