Bruker's MuVi Selective-Plane Illumination Microscope (SPIM) is the fastest multiview system on the market. MuVi incorporates years of Luxendo light-sheet technology innovations to provide high-speed volumetric acquisition for analyzing dynamic processes in delicate live specimens, as well as offering compatibility with any clearing method.
With its highly modular concept, the MuVi SPIM facilitates either live-sample (LS) or cleared-sample (CS) imaging simply by exchanging the acquisition unit. A variety of other advanced hardware and software integrations can be easily configured — often in the field — to support unique and evolving multidimensional experimental needs.
MuVi SPIM features a unique 4-axis concept and is available in various configurations to enable:
The MuVi SPIM is equipped with a motorized sample holder which enables sample rotation by 360°. Although sample rotation is not required to achieve in-toto imaging, a single 90° rotation of the sample can provide isotropic resolution and improve image quality. This is at least four times faster compared to conventional SPIM.
Built on Bruker's history of innovation, our next-generation MuVi SPIM incorporates several new features to enhance image quality, shorten acquisition speed, and allow high-resolution and artifact-free imaging of a broader range of samples.
MuVi SPIM obtains large FOV images at high 3D spatial resolution by using a tightly focused Gaussian beam swept at a high speed along the illumination axis. Integration over time and space leads to an elongated, uniform, and thin light-sheet to provide uniform axial resolution over large fields of view.
When imaging thicker samples or opaque specimens, light scattering can lead to reduced contrast and image blur. MuVi SPIM has implemented an elegant approach to enhance image contrast by offering:
When imaging live, cleared, or fixed biological samples, obstacles like pigments or cell nuclei can absorb or scatter light, leading to images with shadows and striping artifacts. The next-generation MuVi SPIM employs a dual de-striping via pivot scanning, which generates a homogenous illumination profile without compromising acquisition speed.
The sample chamber and all objective lenses are mounted on the octagon, the optical core unit that holds all the objective lenses.
Different octagons can support different magnifications and objective NAs and are compatible with objectives optimized for either live or cleared sample imaging.
The octagon can be easily removed and replaced while maintaining its optical alignment.
Equipped with a full environmental control module, MuVi SPIM supports imaging of various live specimens over hours and days without altering their biology. By enabling precise control over temperature (20-37 °C), gas concentration (CO2, O2, N2), and humidity, MuVi SPIM delivers unique advantages for research in the fields of:
With its exceptional imaging speed, the MuVi SPIM can capture many dynamic biological events. This makes it:
The ability to spatiotemporally control, manipulate, and alter biological processes is key to revealing relevant mechanisms or mechano-chemical interplay. A photomanipulation (PM) module can be added to MuVi SPIM, which allows for advanced experiments in photoablation, FRAP, optogenetics, and cauterization with:
Used in combination with optical clearing techniques, MuVi SPIM enables comprehensive and non-destructive visualization of very large, in-toto cleared specimens within minutes. This is particularly useful for studying:
MuVi SPIM can image a broad range of cleared samples with different sizes, ranging from 3D organoids to large tissue samples and up to the size of a cleared mouse brain. This is accomplished by:
MuVi SPIM provides an innovative solution for sample mounting, minimizing users' exposure to clearing reagents. This is accomplished by:
Illuminations | Detection | Effective Magnification | Field of View (µm) | Pixel Size (nm) | Optical Resolution (nm) |
---|---|---|---|---|---|
10x / 0.3 NA | Olympus 20x / 1.0 NA | 16.7x | 800 | 390 | 300 |
22.2x | 600 | 292 | |||
33.3x | 400 | 195 | |||
44.4x | 300 | 146 | |||
10x / 0.3 NA | Nikon 16x / 0.8 NA | 12x | 1100 | 542 | 375 |
16x | 830 | 406 | |||
24x | 550 | 271 | |||
32x | 415 | 203 |
Illuminations | Detection | Effective Magnification | Field of View (µm) | Pixel Size (nm) | Optical Resolution (nm) |
---|---|---|---|---|---|
10x / 0.3 NA | Nikon 20x / 1.0 NA | 15x | 890 | 434 | 300 |
20x | 665 | 325 | |||
30x | 445 | 217 | |||
40x | 333 | 162 | |||
10x / 0.3 NA | Nikon 10x / 0.5 NA | 7.5x | 1775 | 867 | 600 |
10x | 1330 | 650 | |||
15x | 890 | 434 | |||
20x | 665 | 325 | |||
4x / 0.13 NA | Olympus 4x / 0.28 NA | 2.2x | 6600 | 2960 | 1100 |
4.4x | 3300 | 1480 | |||
6.6x | 1996 | 980 | |||
8.8x | 1515 | 740 |
Bruker's LuxControl software saves time and enhances productivity by providing:
MuVi SPIM records a sample from different angles/views and generates images composed of multiple tiles. The LuxControl software ensures high-quality, 360° crisp images of the sample that compensate for absorption and scattering. Features include:
LuxControl's integrated 3D data viewer allows researchers to inspect the entire dataset directly after acquisition. This gives users control over their data with key capabilities, including: