ELEXSYS E580

多周波数

E580は、標準のXバンドからL、S、Qバンドへのアップグレードを容易にするIF（中間周波数）設計を採用しています。

0.5 ns

SpecJet-IIIデジタイザーとVAMP-IIIビデオアンプは、1GHzの非常に広い検出帯域幅を実現しています。

shaped pulse

SpinJet AWG (Arbitrary Waveform Generator) では、感度と選択性が向上したshaped pulseを自在に扱えます。

ELEXSYS E580, the ultimate EPR System for Life Science, Materials Science and Quantum Computing

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ELEXSYS E580Q Spectrometer Installation

ELEXSYS E580Q Spectrometer Installation at the BioEmPiRe Centre for Structural Biological EPR Spectroscopy, University of Manchester – From Consultation to Operation

Bruker provides comprehensive support throughout the entire EPR installation journey – from the early stages of consultation and infrastructure planning to delivery, installation and final training. This end-to-end approach ensures that each system is seamlessly integrated into the research environment and fully aligned with the user’s scientific goals.

Our close collaboration with researchers worldwide enables us to tailor every installation to the specific needs of each institution, delivering both technical precision and long-term operational confidence.

An example is the installation of the ELEXSYS E580Q Spectrometer at the University of Manchester.

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ELEXSYS E580Q Spectrometer Installation at the BioEmPiRe Centre for Structural Biological EPR Spectroscopy, University of Manchester

特長

ESRスペクトルと電子スピン緩和時間(T1,T2)の基本的な検出器としてのFT-ESRおよび電子スピンエコー技術
ESEEMと2D-HYSCOREによる電子と核スピンの超微細相互作用によるスピン密度分布と距離の測定
超微細相互作用と核スピン緩和を測定するためのPulse-ENDOR
電子・電子スピン双極子結合による長距離測定のためのPulse-ELDORおよびDEER
相関関係と交換比率を測定するためのSECSYとEXSY
レーザーを用いた化学反応や三重項状態を評価する実験

アプリケーション

SECSY and EXSY

Spin Echo Correlation Spectroscopy (SECSY) and Exchange Spectroscopy (EXSY) are 2-dimensional techniques. The positions of cross peaks in the 2D spectrum are used to correlate spectral peaks with each other and the cross peak amplitudes carry the information about exchange rates. These experiments are standard features of the E 580 spectrometer and are supported by predefined PulseSPEL programs.

Laser Triggered Experiments

An external event, e.g. a laser flash, can be used to generate a paramagnetic state. For this type of experiment the instrument can be triggered externally or alternatively the instrument can send a trigger pulse to activate an external device. Precise synchronization between the external event, the microwave pulses and the data acquisition is established. Any pulse-EPR experiment can be run in combination with the external trigger function.

Pulse ELDOR and DEER

Electron-Electron Double Resonance (ELDOR) and Double Electron-Electron Resonance (DEER) are acronyms for the same technique. With this technique the dipolar interaction between electron spins is measured. This information directly translates into the distance between the electron spins. Distance ranges of 10 to 80 Angstrom are covered. In recent years the combination of site directed spin labeling and DEER became one of the most popular tools in structural protein research.

ESEEM and 2D-HYSCORE

Electron Spin Echo Envelope Modulation (ESEEM) is one of the most popular techniques to measure the hyperfine interaction (HFI) parameters between electron spins and nearby nuclear spins. From the analysis of the ESEEM signals detailed information about electron spin density distribution, distances and bonding angles is gained. 2D-HYSCORE is a technique also based on the ESEEM effect and is the method of choice for frozen solutions and powders. All ESEEM-type of experiments are standard features of the E 580 and are supported by dedicated acquisition and processing tools in Xepr.

1H HYSCORE of nitroxide spin probe: (right) 166 ns Chirped (± 113 MHz) inversion pulse and (left) 26 ns standard inversion pulse. The excitation bandwidth of the inversion pulses are indicated on the field sweep spectrum, red = chirped and blue = standard.

4-Pulse DEER spectra of spin-labeled Cu containing protein with the pump pulse centered on the nitroxide spectrum (red arrow) and observation in the Cu spectrum (blue arrow). Sample courtesy Thomas Prisner

Pulse ENDOR, a complementary technique to ESEEM, combines both MW and RF pulses. It measures hyperfine interactions (HFI) to give detailed information about electron spin density, distances, and bonding angles. It excels at strong HFI that occur for very short distances or transition metal nuclei.

Substantial increase of sensitivity in DEER experiment at Q‑Band (33.5 GHz, 25 min measuring time) as compared to X‑Band (9.5 GHz, 22 h measuring time).

仕様

パルスESRは常磁性体を調査するためのツールとして定着してきました。1987年に発売したESP 380は、ブルカーが初めて市販したパルスESR装置です。次のランドマークとなったのは、後継機種のELEXSYS E 580と、新しい高速デジタルデバイスのPatternJetとSpecJetです。ハードウェアの制約を受けずに、スピン緩和に支配された速度でFIDとエコーを平均化することが可能となった、史上初の装置です。

アクセサリー

分光計のもう一つの礎であるプローブヘッドの開発には、長年取り組んできました。その結果、伝説的なFlexlineプローブヘッドシリーズが誕生しました。

ELEXSYS E 580 FT/CW X-バンド分光計は、多周波数のための拡張プラットフォームでもあります。X-バンド分光計をベースに、W-バンド（E 680）、Q-バンド（SuperQ-FT）、S-バンド、（SuperS-FT）、L-Band（SuperL-FT）への周波数変換が可能です。独自の設計コンセプトにより、Xバンドの全ての特徴をデュアルバンド分光計の追加周波数に変換することができます。

SpinJet-AWG II

Arbitrary Waveform Generators (AWGs) are integral to modern pulse EPR spectrometers. With the introduction of the second generation of the SpinJet-AWG, Bruker is taking the next step in shaping EPR experiments. With updated software and advanced hardware components, the SpinJet-AWG II is the ideal product for pushing the frontiers of your EPR research, and has the following features and specifications:

WURST, sech/tanh, and Fourier-series as new pre-defined pulse shapes
Phase cycling of custom pulse shapes
2.0 GS/s, 0.5 ns resolution
14-bit amplitude resolution
0.5° phase resolution
1 GHz bandwidth
Increased memory with optimized usage
Compatible with all frequency bands offered by Bruker
Calibration table for optimized suppression of the carrier frequency
The SpinJet-AWG II is available with all new ELEXSYS E580 systems and as an upgrade to your current system

Clean HYSCORE spectrum of coal recorded with the SpinJet-AWG II

ソフトウェア

実験デザインとデータ処理のためのXeprソフトウェア

これまでにない柔軟性と操作性が、Xeprソフトウェアの特長です。単純なエコー減衰実験でも、複雑なマルチパルス二次元実験でも、Xeprのグラフィカルユーザーインターフェースが、装置の制御、実験の設定および実行を容易にします。数多くの新しい処理ツール、表示機能、データ表示は、データ解析にも新しい次元を開きます。