Increased endothelial permeability at the beginning of atherosclerosis is believed to allow lipid deposition and plaque formation to occur. At later stages it is associated with plaque inflammation and extravasation of inflammatory cells that occurs as a plaque progresses, and may eventually lead to plaque rupture. This webinar will describe the application of magnetic resonance imaging (MRI) for the characterization of increased endothelial permeability in mouse atherosclerotic plaques using the preclinical Bruker Biospec 7 Tesla horizontal 70/30 scanner and self-gating features (IntraGate). Self-gated imaging allows high-quality cardiac MRI with no additional hardware requirements (such as the use of ECG electrodes) and potential pitfalls of signal interferences and disruption, and has been used in our laboratory to obtain clear imaging of the mouse aortic root.
The basics self-gated imaging, along with the advantages of applying this method in both cardiac and vascular MRI, will be discussed. For example, this flexible tool allows high quality, high resolution pre-clinical cardiac and vascular imaging without the need for ECG triggering, meaning no electrodes and no gating with a device is needed.
The webinar will be useful to anybody interested in cardiac and vascular imaging of small animals, particularly using MRI. It would also be useful for researchers who image larger animals on clinical scanners but who are interested in smaller animal imaging where more indepth mechanistic studies can be conducted. The webinar will also appeal to anyone interested in the pathology of cardiovascular disease.
Claudia Calcagno Mani, MD, PhD
Assistant Professor Department of Radiology Translational and Molecular Imaging Institute Icahn School of Medicine at Mount Sinai New York, USA
Todd Sasser, PhD
Head of Applications PCI, Americas Bruker BioSpin Billerica, Massachusetts