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NeuraLight 3D SLM for Ultima

The Ultima NeuraLight 3D™ is the next step forward in the use of optogenetics in functional paradigms to study and understand neural connectivity and how neural networks function. In close collaboration with leaders in neuroscience research at Stanford University, Columbia University, and University College London, Bruker has developed the next major technological breakthrough to complement the ever evolving Ultima family of multiphoton microscopes.

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Opterra II Live-Cell Imaging

3D Optical Stimulation and Multiphoton Imaging Combined

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Optical stimulation of selected cells containing GCaMP6s using NeuraLight 3D. Cell bodies were stimulated simultaneously by creating a 3D hologram of points with the SLM and spiral scanning all points over the cell body. Courtesy of Adam Packer, Lloyd Russell, Henry Dalgleish, and Michael Hausser (University College London). 

Titrate precise, simultaneous, multicell stimulation in a 3D volume for in vivo studies.

The Ultima NeuraLight 3D SLM (spatial light modulator) leverages the flexibility of Ultima’s modular platform design, along with new licensed technology, to create 3D laser holograms to simultaneously stimulate cells in three dimensions. Coupled with a set of 6-millimeter galvanometers, the NeuraLight 3D SLM enables multiple stimulation points in either spiral or rectangular scan patterns to be projected in a 3D volume.

Perform multiplexed diffraction-limited subcellular photoactivation.

NeuraLight 3D can also generate precise, diffraction-limited points in a 3D space for subcellular resolution. Stimulation patterns targeting individual synapses or groups of synapses can be produced, allowing researchers to probe neural connectivity and signaling. Sequencing of stimulation patterns enables researchers to automate studies examining signal summation in individual neurons or in groups of neurons.

Target Functional Ensembles of Neurons

Using the NeuraLight 3D SLM, multiple targeted spots are generated and then spun in controlled spirals, creating a swinging spot grid for simultaneous optical stimulation and imaging.

Rapidly switch between stimulation patterns.

The fast, 300-hertz (upgradable to 500-hertz) switching time of the NeuraLight 3D SLM allows users to rapidly change stimulation patterns. For in vivo studies, this provides a tool to mimic neuronal firing produced by behavioral stimuli. For subcellular studies examining connectivity and signaling, complex signal summation studies can be quickly executed.

Generate 3D activation patterns while imaging and recording electrical signals.

The module includes a complete software toolkit for generating 3D activation patterns while imaging/recording electrical signals and triggering behavioral and electrical stimuli. A calibration wizard makes calibration a simple and straight-forward process. 3D point location is mapped using an acquired Z series of the specimen. This also allows synchronization with imaging (resonant scanning, scanning line scanning, and point detection), along with electrical recording and triggering of other stimuli, including electrical and behavioral stimuli.

Opterra II Live-Cell Imaging

Add NeuraLight 3D to your Ultima system

The NeuraLight 3D is available as an option on new Ultima In Vitro and Ultima IntraVital multiphoton systems. It is also available as an upgrade to Ultima systems produced since 2006.

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Watch our Ultima NeuraLight 3D Webinar

Learn how pairing a Spacial Light Modulator(SLM) and a Multiphoton Microscope can enable simultaneous optical stimulation and optical recording to study neural networks.