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The BioScope Resolve with NEW Advanced Correlation Stage

How Correlative Microscopy Should Be

  • True synchronization of AFM and light microscopy across the entire microscope stage – for the first time!
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  • Highest AFM resolution performance over the widest range of sample sizes, from single molecules to cells and tissues
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  • Simple ‘Click & Go’ operation for fast and easy experimental setup
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  • Enhanced productivity for mapping large sample areas (over mm’s) through automation of all measurements
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  • Fully correlated datasets collected in situ and in real-time under physiologically relevant conditions

 

 

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BioScope Resolve AFM 052118

Advancing Your Bioresearch to the Next Stage

DNA DbHelix ACStage Final

Major and minor grooves of the DNA double helix. Image captured using the Advanced Correlation Stage integrated with an inverted light microscope. Scalebar: 25nm

Introducing the new Advanced Correlation Stage for the ultimate integration of AFM with optical microscopy. Enhanced flexibility provides true correlated datasets across the entire microscope stage while maintaining full compatibility with optical techniques. The same unrivaled performance of the BioScope Resolve for high-resolution imaging and biomechanics studies – now even better.

True Correlative AFM and Optical Microscopy

The Advanced Correlation Stage extends the AFM-accessible sample area to match the larger field of view of the optical microscope. Accurate AFM measurements can now be conducted over the entire microscope stage thus providing true correlated mapping over large distances (millimeters).

Together with Resolve’s MIROView software interface, tiling of AFM and optical images is easily conducted. Image tiling is often required for capturing high-resolution images of large biological samples, such as tissues, or for collecting a statistically relevant sample size of mechanical property measurements on individual mammalian cells.

Tiling Overlay AnimatedGIF 1

Tiling of both phase contrast and FastForce Volume images over a 900µm x 900µm area of living cells using the Advanced Correlation Stage. Optical = greyscale; Topography = gold color scale; Modulus = purple color scale.

Automated Hi-Res Mapping at Relevant Timescales

AdvancedCorrelationStage FastTapping HiResPhaseImage ScaleBar 2

 

 

Image tiling enables data collection over large sample areas while maintaining high-resolution images. This, however, results in much larger datasets that take longer times to acquire. When studying changes in the structure or mechanical properties of biological samples like mammalian cells, high-resolution data must be collected on timescales relevant to these biological processes.

By combining the Advanced Correlation Stage with Resolve’s Fast Tapping imaging mode, for the first time, automated high-resolution maps of multiple living cells can be collected over millimeter-sized fields of view in the same amount of time that it takes a traditional AFM system to collect a single image.

 

 

 

 

 

 

High-resolution mapping of living MDCK cells. Individual 90µm AFM images were collected at high pixel density (1024 x 1024) over a 400µm area (bottom image = phase channel). At ~8min/frame, the total time to capture all images was ~72min. Data courtesy of Dr. Hermann Schillers, University of Münster, Germany.

Correlative Microscopy Has Never Been Easier!

With MIROView’s intuitive workflow, the Advanced Correlation Stage provides quick and easy experimental setup with simple ‘Click & Go’ operation. Mode switching offers total flexibility for collecting multiparametric datasets while automated collection of all data results in significantly enhanced productivity. For the first time, synchronization between AFM and light microscopy can be conducted across the entire microscope stage ensuring that only the highest quality data is collected - how true correlative microscopy should be!

 

 

Learn more about BioScope Resolve

The Advanced Correlation Stage provides ultimate flexibility and enhanced productivity for conducting correlated microscopy studies across the entire microscope stage.